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Documentos Principais

SRP2028

Sigma-Aldrich

TFIIA, reconstituted human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

Sinônimo(s):

MGC129969, TF2A1, TF2A2, TFIIA

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.26

fonte biológica

human

recombinante

expressed in E. coli

Ensaio

≥80% (SDS-PAGE)

forma

frozen liquid

peso molecular

66 kDa

embalagem

pkg of 10 μg

condição de armazenamento

avoid repeated freeze/thaw cycles

concentração

150 μg/mL

cor

clear colorless

nº de adesão NCBI

NM_004492
NM_015859

nº de adesão UniProt

P52655

Condições de expedição

dry ice

temperatura de armazenamento

−70°C

Informações sobre genes

human ... GTF2A1(2957) , GTF2A2(2958)

Ações bioquímicas/fisiológicas

The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α subunit), 19 kDa (β subunit) and 12 kDa (γ subunit). The α and β subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ subunit), retains native TFIIA activity.

forma física

Clear and colorless frozen liquid solution

Nota de preparo

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

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J A Ranish et al.
The Journal of biological chemistry, 266(29), 19320-19327 (1991-10-15)
The general transcription factor TFIIA was purified from yeast. A key step in the purification was affinity chromatography using a column containing the adenovirus major late promoter with bound recombinant TFIID to which TFIIA binds with high affinity. TFIIA activity
S Buratowski et al.
Cell, 56(4), 549-561 (1989-02-24)
A native gel electrophoresis DNA binding assay was used to resolve complexes formed on the adenovirus Major Late Promoter by general transcription factors and RNA polymerase II. Five sets of complexes containing distinct components were identified. These complexes were generated
J DeJong et al.
Genes & development, 7(11), 2220-2234 (1993-11-01)
TFIIA is a transcription factor that, by interacting with the TATA-binding subunit (TBP) of TFIID, modulates transcription initiation by RNA polymerase II in vitro. By use of a mobility shift assay, TFIIA was purified from HeLa cells as a complex

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