SRP0127

DNMT2 Active human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Sinônimo(s): DNA (cytosine-5-)-methyltransferase 2, PUMET, RNMT1, tRNA (cytosine-5-)-methyltransferas, tRNA aspartic acid methyltransferase 1
• Código UNSPSC: 12352200
• NACRES: NA.32

Propriedades

• fonte biológica: human
• recombinante: expressed in baculovirus infected insect cells
• Ensaio: ≥80% (SDS-PAGE)
• Formulário: aqueous solution
• peso molecular: 71 kDa
• embalagem: pkg of 10 μg
• concentração: >0.02 mg/mL
• nº de adesão NCBI: NM_004412
• nº de adesão UniProt: Q6ICS7
• Condições de expedição: dry ice
• temperatura de armazenamento: −70°C
• Informações sobre genes: human ... TRDMT1(1787)

Descrição

Descrição geral

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) is a highly conserved protein which is part of the DNA methyltransferase family. It possesses a conserved cysteine residue in its catalytic pocket. The gene encoding it is localized on human chromosome 10p14->p12.

Aplicação

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Ações bioquímicas/fisiológicas

DNA (cytosine-5-)-methyltransferase 2 (DNMT2) methylates the cytosine-38 residue of the aspartic acid transfer RNA (tRNA-Asp). It has been shown to interact with the anticodon stem and loop. The protein also functions in cellular physiology and stress response. It is significantly expressed in cancers.

forma física

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Nota de preparo

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.