SAB4702136

Monoclonal Anti-CD3 APC-Cyanine7 produced in mouse

• Sinônimo(s): CD3E, T3E, TCRE
• Código UNSPSC: 12352203

Propriedades

• conjugado: APC-Cy7 conjugate
• Nível de qualidade: 100
• tipo de produto de anticorpo: primary antibodies
• clone: SK7, monoclonal
• forma: buffered aqueous solution
• reatividade de espécies: ( Human), (Non-human primates)
• técnica(s): flow cytometry: suitable
• Isotipo: IgG1κ
• nº de adesão UniProt: P07766
• temperatura de armazenamento: 2-8°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CD3E(916)

Descrição

Descrição geral

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

Especificidade

The mouse monoclonal antibody SK7 recognizes an extracellular epitope on the CD3 antigen on mature human T cells. This antibody reacts with the epsilon chain of the CD3 complex. The monoclonal antibodies SK7 and UCHT1 recognize overlapping epitopes.

Imunogênio

Human thymocytes

forma física

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Nota de preparo

Purified antibody is conjugated with activated tandem dye of allophycocyanin-cyanine 7 (APC-Cy7) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Armazenamento e estabilidade

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Outras notas

The antibody solution should be gently mixed before use.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable