SAB4200839

Anti-Acetylcholinesterase (AChE) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• Sinônimo(s): AChE
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• forma do anticorpo: IgG fraction of antiserum
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• forma: buffered aqueous solution
• reatividade de espécies: human
• embalagem: antibody small pack of 25 μL
• concentração: ~1 mg/mL
• técnica(s): immunoblotting: 1:1000-1:2000 using whole extracts of Jurkat cells.; immunofluorescence: 1:1000-1:20000 using Human A431
• nº de adesão UniProt: P22303
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... ACHE(43)

Descrição

Descrição geral

Acetylcholinesterase (AChE) is a member of the a/ß-hydrolase-fold proteins superfamily which is composed of structurally related proteins with a great diversity in their catalytic, recognition, adhesion and chaperone functions. 3D structure studies of a/ß-hydrolase reviled a variety of folding patterns suggesting associations through oligomerization dependent functions.1 AChE is a tissue specific serine hydrolase expressed in several variants at neuromuscular junctions (NMJs)2, cholinergic brain synapses2-3 or erythrocyte (Red Blood Cells- RBC)4.

Especificidade

Anti-Acetylcholinesterase (AChE) antibody specifically recognizes human AChE.

Imunogênio

human Acetylcholinesterase, recombinant, expressed in HEK 293 cells

Aplicação

The antibody may be used in various immunochemical techniques including Immunoblotting Immunofluorescence and ELISA. Detection of the AChE band by Immunoblotting is specifically inhibited by the immunogen.

Ações bioquímicas/fisiológicas

In the synaptic cleft, AChE main function is to ensure rapid hydrolysis of acetylcholine (ACh) released from motor nerve ending into choline and acetic acid thus preventing postsynaptic muscle ACh receptors (AChR) hyperactivation and termination of the signal transmission.2-3,5AChE and Butyrylcholinesterase (BChE) inhibitory drugs or peptides are being used in several neurodegenerative diseases including Alzheimer′s disease and pathological muscle weakness such as Myasthenia Gravis. These AChE inhibitors increase synaptic ACh levels and hence improve the cholinergic function in the brain.2-3,5-6 Erythrocytes AChE (AChE-H) is a membrane encored AChE splicing variant found in RBC, it belongs to the glycosylphosphatidylinositol (GPI) protein family and carries the Yta blood group antigen. Erythrocytes AChE can be found in plasma circulating vesicles originated from aged erythrocytes, and therefore is considered as a biomarker for RBC membrane integrity. Furthermore, Erythrocytes AChE activity is also a marker for inflammation and other diseases including essential hypertension, glaucoma, ALS, neurotoxicity, and Hirschsprung′s disease.4,7-8

forma física

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Armazenamento e estabilidade

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable