SAB4200798

Anti-GSDMD antibody, Mouse monoclonal

clone GSD-14, purified from hybridoma cell culture

• Sinônimo(s): Anti-Gasdermin domain-containing protein 1, Anti-Gasdermin-D
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: GSD-14, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: ~53 kDa
• reatividade de espécies: human
• concentração: ~1 mg/mL
• técnica(s): immunoblotting: 2-4 μg/mL using human Jurkat cell extract; immunofluorescence: 5-10 μg/mL using human A431 cells
• Isotipo: IgG1
• nº de adesão UniProt: P57764
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... GSDMD(79792)

Descrição

Descrição geral

GSDMD (gasdermin D) also known as DFNA5L or Gasdermin domain-containing 1 (GSDMDC1), belongs to the gasdermin protein family of epithelial proliferation regulators. GSDMD is expressed in the upper gastrointestinal epithelium differentiating cells and has been suggested to act as a tumor suppressor since it is suppressed in high percentage of esophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gramnegative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1β processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). It was also reported that Caspase-4/11 specifically cleaves GSDMD after Asp275, leading to gasdermin-N domain that bears intrinsic pyroptosisinducing activity. 4 Furthermore, GSDMD-mediated pyroptosis plays an important role in mature IL-1β release without affecting its maturation.

Monoclonal Anti-GSDMD recognizes human GSDMD.

Monoclonal Anti-GSDMD (mouse IgG1 isotype) is derived from the GSD-14hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with synthetic peptide corresponding to the internal region of human GSDMD, conjugated to keyhole limpet hemocyanine (KLH). Gasdermin D (GSDMD) is also known as DFNA5L or gasdermin domain-containing 1 (GSDMDC1). It belongs to the gasdermin protein family of epithelial proliferation regulators and is expressed in the upper gastrointestinal epithelium differentiating cells.

Imunogênio

Synthetic peptide of human GSDMD, conjugated to KLH

Aplicação

The antibody is recommended to use in various immunological techniques, including Immunoblot (∼53 kDa) and Immunofluorescence.

Ações bioquímicas/fisiológicas

Gasdermin D (GSDMD) is known to act as a tumor suppressor since it is suppressed in a high percentage of oesophageal squamous cell carcinomas and gastric cancers. In the presence of lipopolysaccharide (LPS) from Gram-negative bacteria, inflammatory caspases including caspase-4/11 activates downstream pyroptotic cell death, interleukin-1 processing, and lethal septic shock. The absence of GSDMD, completely blocked LPS electroporation-triggered pyroptosis in studies performed using GSDMD siRNA knockdown or GSDMD knockout (both human HeLa cells and iBMDM mouse cells). Furthermore, GSDMD-mediated pyroptosis plays an important role in mature interleukin-1 (IL-1) release without affecting its maturation.

forma física

Supplied as a solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Outras notas

This product is for R&D use only, not for drug, household, or other uses.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 3