SAB4200562

Anti-AOX1 antibody, Mouse monoclonal

clone AO15, purified from hybridoma cell culture

• Sinônimo(s): Monoclonal Anti-AO, Monoclonal Anti-AOH1, Monoclonal Anti-AOX1 antibody produced in mouse, Monoclonal Anti-EC 1.2.3.1, Monoclonal Anti-aldehyde oxidase 1
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: AO15, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~148 kDa
• reatividade de espécies: human
• concentração: ~1 mg/mL
• técnica(s): western blot: 1.0-2.0 μg/mL using extracts of HepG2 cells.
• Isotipo: IgG1
• nº de adesão UniProt: Q06278
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... AOX1(316)

Descrição

Descrição geral

Monoclonal Anti-AOX1 (mouse IgG1 isotype) is derived from the hybridoma AO15 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide. Aldehyde oxidase 1 (AOX1) comprises the 2Fe/2S clusters and two flavin adenine nucleotide (FAD) domain-containing N-terminal domain I and II, respectively. The C-terminal domain encompasses domain III with a substrate-binding site. The gene encoding AOX1 is mapped to human chromosome 2q33.1.

Especificidade

Monoclonal Anti-AOX1 recognizes human AOX1.

Imunogênio

synthetic peptide corresponding to an internal sequence of human AOX1. The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

Aplicação

Monoclonal Anti-AOX1 antibody produced in mouse has been used in reverse-phase protein array analysis(5) and immunoblotting.

Ações bioquímicas/fisiológicas

Aldehyde oxidase 1 (AOX1), along with the endoplasmic cytochrome P450 system (CYP450), participates in the drug metabolism and generation of reactive oxygen species. It also has a role in the hepatic phase I metabolism of numerous xenobiotics. Reduced AOX1 protein expression is implicated in chronic pancreatitis, and hepatocellular carcinoma. Furthermore, hypermethylation of the AOX1 promoter region is observed in colon cancer and prostate cancer, making it a potential biomarker for prostate cancer diagnosis. Polymorphism in the AOX1 gene also displays variation in metabolism of the quinoxaline phenoxypropionic acid derivative XK469.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing,or storage in “frost-free” freezers,isnot recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable