SAB4200369

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse

clone R2.5254.2, purified from hybridoma cell culture

• Sinônimo(s): Anti-CD112, Anti-HVEB, Anti-PRR2, Anti-PVRL2, Anti-PVRR2, Anti-Poliovirus receptor-related 2 (herpesvirus entry mediator B)
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: R2.5254.2, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~72 kDa
• reatividade de espécies: rat, human, monkey, mouse
• concentração: ~1.0 mg/mL
• técnica(s): flow cytometry: 5-10 μg/test using using 1x10⁶ MDA-MB-231 cells; immunocytochemistry: 2.5-5 μg/mL using using MDA-MB-231 cells; indirect ELISA: suitable; western blot: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... NECTIN2(5819) , PVRL2(5819) , PVRL2(308417) ; mouse ... Nectin2(19294)

Descrição

Descrição geral

Monoclonal Anti-Nectin-2/PVRL2 (mouse IgG1 isotype) is derived from the hybridoma R2.525.2 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with 3T3 cells expressing human nectin-2. Nectin-2 is found to be widely expressed in human tissues, including hematopoietic cells.

Nectin-2, also known as poliovirus receptor related protein 2 gene (PRR2), is encoded by the gene mapped to human chromosome 19q13.2. The encoded protein belongs to the nectin protein family and is characterized with an extracellular region with three immunoglobulin-like loops, a single transmembrane domain and a cytoplasmic domain.

Especificidade

The antibody has been reported to block cell adhesion and herpes virus entry.

Imunogênio

3T3 cells expressing human Nectin-2.

Aplicação

Monoclonal Anti-Nectin-2/PVRL2 antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• immunocytochemistry
• flow cytometry

Ações bioquímicas/fisiológicas

Nectins act as key elements of adherens junctions and facilitate their formation. Nectin-2 possesses anti-viral property against herpes simplex virus (HSV)-2 infection. In mice, loss of nectin-2 affects sperm production and male-specific infertility. Aberrations in the gene has been associated with the pathogenesis of peripheral T-cell lymphoma (PTCL). Serum levels of nectin-2 can be used as a diagnostic and prognostic marker for colorectal cancer (CRC).

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable