SAB4200107

Anti-CHD4 antibody, Mouse monoclonal

clone 3F2/4, purified from hybridoma cell culture

• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: 3F2/4, monoclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~250 kDa
• reatividade de espécies: human, mouse
• concentração: ~1.0 mg/mL
• técnica(s): immunoprecipitation (IP): suitable; western blot: 0.5-1.0 μg/mL using HeLa cell extracts
• Isotipo: IgG2a
• nº de adesão UniProt: Q14839
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CHD4(1108)

Descrição

Descrição geral

Monoclonal Anti-CHD4 (mouse IgG2a isotype) is derived from the hybridoma 3F2/4 produced by the fusion of mouse myeloma cells and splenocytes from CD-1 mice immunized with a synthetic peptide derived from human chromodomain helicase DNA binding protein 4 (CHD4) conjugated to keyhole limpet hemocyanine (KLH). CHD4 is one of the subunits of Mi-2, an ATP-dependent helicase. It belongs to the sucrose non-fermentable (SNF2)/Rad54 helicase family of proteins. The CHD4 gene is mapped to human chromosome 12p13.31 and is highly conserved among animals and plants.

Imunogênio

synthetic peptide derived from human CHD4, conjugated to KLH

Aplicação

Monoclonal Anti-CHD4 antibody produced in mouse has been used in:

• immunoblotting
• enzyme linked immunosorbent assay (ELISA)
• immunoprecipitation

Ações bioquímicas/fisiológicas

Chromodomain helicase DNA binding protein 4 (CHD4) interacts with metastasis-associated protein (MTA1/2), histone deacetylase 1 (HDAC1) and RbAp46 in the nucleosome remodelling and deacetylation (NuRD) complex. Interestingly, antibodies against CHD4 have been found to be involved in the onset of dermatomyositis (DM), an autoimmune disease which is a form of idiopathic inflammatory myopathy (IIM). Variation in the gene due to mutation is observed in endometrial cancer. CHD4 regulates homologous recombination and thus maintains genome stability.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable