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Key Documents

SAB4200002

Sigma-Aldrich

Anti-POU1F1/PIT1 antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

Sinônimo(s):

Anti-GHF-1, Anti-Pituitary-specific positive transcription factor 1

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

rabbit

conjugado

unconjugated

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

forma

buffered aqueous solution

peso molecular

antigen ~32 kDa

reatividade de espécies

human

concentração

~1.0 mg/mL

técnica(s)

immunoprecipitation (IP): 2.5-5 μg using GH3 cell lysates
western blot: 1-2 μg/mL using GH3 cell lysates

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... POU1F1(5449)

Categorias relacionadas

Descrição geral

POU1F1 is encoded by the gene mapped to human chromosome 3p11.2.
Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is a pituitary-specific transcription factor and is a member of the POU family of transcription factors.

Especificidade

Anti-POU1F1/PIT1 recognizes human POU1F1/PIT1.

Aplicação

Anti-POU1F1/PIT1 antibody produced in rabbit has been used in immunoblotting and immunoprecipitation.

Ações bioquímicas/fisiológicas

Pituitary-specific positive transcription factor 1 (POU1F1/PIT1) is responsible for pituitary development and hormone expression in mammals. It regulates mammalian development. POU1F1/PIT1 is required for the differentiation and proliferation of the anterior pituitary somatotrophs, lactotrophs and thyrotrophs cell lineages. Mutation in Pit-1 gene is associated with the syndrome of combined pituitary hormone deficiency (CPHD), a disease characterized by the lack of prolactin, growth hormone and thyroid stimulating hormone β. POU1F1/PIT1 regulates its target gene expression by binding to response elements on their promoter regions and recruitment of co-regulatory proteins, that alter histone acetylation and modify chromatin structures.

forma física

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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Visite a Biblioteca de Documentos

Pituitary transcription factors in the aetiology of combined pituitary hormone deficiency
Pfaffle R and Klammt J
Best Practice & Research. Clinical Endocrinology & Metabolism, 25(1), 43-60 (2011)
Shusuke Akamatsu et al.
Nature genetics, 44(4), 426-429 (2012-03-01)
We have previously reported multiple loci associated with prostate cancer susceptibility in a Japanese population using a genome-wide association study (GWAS). To identify additional prostate cancer susceptibility loci, we genotyped nine SNPs that were nominally associated with prostate cancer (P
Evolutionary and functional analysis of the key pluripotency factor Oct4 and its family proteins
Zhang X, et al.
Journal of Genetics and Genomics = Yi Chuan Xue Bao, 40(8), 399-412 (2013)
The Pit-1beta domain dictates active repression and alteration of histone acetylation of the proximal prolactin promoter
Diamond SE and Gutierrez HA
The Journal of Biological Chemistry, 275(40), 30977-30986 (2000)
Growth rate and carcass quality in pigs as related to genotype at loci POU1F1/RsaI (Pit1/RsaI) and GHRH/AluI
Pierzchala M, et al.
Animal Science Journal = Nihon Chikusan Gakkaiho, 21(3), 159-166 (2003)

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