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Documentos Principais

P9498

Sigma-Aldrich

Monoclonal Anti-PIAS-x antibody produced in mouse

clone PIASX-116, purified immunoglobulin, buffered aqueous solution

Sinônimo(s):

Anti-Protein Inhibitor of Activator STAT

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.41

fonte biológica

mouse

conjugado

unconjugated

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

PIASX-116, monoclonal

forma

buffered aqueous solution

peso molecular

antigen ~75 kDa

reatividade de espécies

human

técnica(s)

immunocytochemistry: suitable
indirect ELISA: suitable
microarray: suitable
western blot: 1-2 μg/mL using proteasome fraction II of HeLa cells

Isotipo

IgG1

nº de adesão UniProt

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... PIAS2(9063)

Descrição geral

Monoclonal Anti-PIAS-x (mouse IgG1isotype) is derived from the PIASX-116 hybridoma produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice. Protein inhibitor of activated STAT 2 (PIAS2/PIAS-x), a small ubiquitin-like modifier (SUMO) E3 ligase contains a zinc binding motif and a highly acidic region. This gene is located on human chromosome 18q21.1.

Especificidade

Monoclonal Anti-PIAS-x recognizes human PIAS-x.

Imunogênio

peptide corresponding to amino acids 26-40 of human PIAS-x.

Aplicação

Monoclonal Anti-PIAS-x antibody produced in mouse may be used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunocytochemistry
  • immunoblotting

Ações bioquímicas/fisiológicas

Protein inhibitor of activated STAT2 (PIAS2) plays a key role in SUMO modification (SUMOylation) of several host and viral proteins. PIAS-x acts as a transcriptional co-repressor of STAT4. It may participate in controlling the chromatin structure. Upregulation or suppression of this gene can control the stability of the hepatitis C virus (HCV) core, NS3 and NS5A proteins. Hence PIAS-x is considered as the restriction factor for HCV replication.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is also not recom-mended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Protein inhibitor of activated STAT2 restricts HCV replication by modulating viral proteins degradation
Guo J, et al.
Viruses, 9(10), 285-285 (2017)
Ulf R Klein et al.
Molecular biology of the cell, 20(1), 410-418 (2008-10-24)
The ubiquitin-like SUMO system controls cellular key functions, and several lines of evidence point to a critical role of SUMO for mitotic progression. However, in mammalian cells mitotic substrates of sumoylation and the regulatory components involved are not well defined.

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