P0085

Anti-Podoplanin antibody, RAT monoclonal

clone NZ-1.2, purified from hybridoma cell culture

• Sinônimo(s): Anti-AGGRUS, Anti-GP36, glycoprotein, 36-KD, Anti-GP40, Anti-Gp38, Anti-OTS8, Anti-T1A, Anti-T1A-2
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• conjugado: unconjugated
• forma do anticorpo: purified from hybridoma cell culture
• tipo de produto de anticorpo: primary antibodies
• clone: NZ-1.2, monoclonal
• forma: buffered aqueous solution
• peso molecular: antigen ~36 kDa
• reatividade de espécies: human
• concentração: ~1.5 mg/mL
• técnica(s): flow cytometry: suitable; immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: 4-8 μg/mL using U87 total cell extract
• nº de adesão UniProt: Q86YL7
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PDPN(10630)

Descrição

Descrição geral

Monoclonal Anti-Podoplanin (rat IgG2a isotype) is derived from the hybridoma NZ-1.2 produced by the fusion of mouse myeloma cells and splenocytes from CD rat immunized with platelet-aggregation-stimulating (PLAG) domain of podoplanin. Podoplanin (Aggrus) is an integral membrane mucoprotein present on the surface of podocytes in kidney glomeruli and the parietal cells of the glomerular Bowman′s capsule. It is a single membrane-spanning protein composed of a heavily glycosylated extracellular domain and a short intracytoplasmic tail. The extracellular domain possesses a platelet aggregation stimulating (PLAG) domain. Podoplanin has been described to be present in normal, inflammatory and neoplastic lymphatic capillary endothelium.

Imunogênio

platelet-aggregation-stimulating (PLAG) domain of human podoplanin.

Aplicação

Anti-Podoplanin antibody, Mouse monoclonal has been used in:

• immunoblotting
• immunoprecipitation
• flow cytometry
• immunocytochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Western Blotting (1 paper)

Ações bioquímicas/fisiológicas

Expression of podoplanin is regulated by the lymphatic-specific homeobox gene Prox-1 (prospero-1). It is involved in lymphatic vessel formation and might be associated with tissue development and repair. Podoplanin is detected in human angiosarcomas, lymphangiomas, and in Kaposi′s sarcomas. It is implicated in the onset of tumor invasion, metastasis or malignant progression. Podoplanin plays an important role in the maintenance of podocyte foot processes and glomerular permeability.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)