MAK277
GAPDH Activity Assay Kit
sufficient for 100 colorimetric tests
Sinônimo(s):
Glyceraldehyde-3-phosphate Dehydrogenase Activity Assay Kit
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.84
Produtos recomendados
método de detecção
colorimetric
doença(s) relevante(s)
cancer
temperatura de armazenamento
−20°C
Descrição geral
GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase; EC 1.2.1.12) catalyzes the conversion of Glyceraldehyde-3-Phosphate (GAP) to 1, 3-Bisphosphate Glycerate (BPG) and plays a key role in glycolysis. The enzyme is involved in cellular processes such as apoptosis, membrane trafficking, iron metabolism and nuclear translocation. GAPDH (housekeeping gene) expression is stable and constitutive. Deregulation of GAPDH activity is associated with abnormal cell proliferation and carcinogenesis. Accurate quantitation of GAPDH activity is important for diagnosing diseases and studying normal cellular physiology.
Aplicação
GAPDH Activity Assay Kit has been used to measure the enzyme activity of glyceraldehyde-3-phosphate dehydrogenase in testis.
Características e benefícios
Compatible with high-throughput handling systems.
Adequação
Suitable for use with various tissue and cell culture samples.
Princípio
The GAPDH Activity Assay Kit provides a simple and sensitive method for monitoring GAPDH activity in various samples. GAPDH activity is determined in a coupled enzyme reaction in which GAP is converted to BPG by GAPDH. This results in a colorimetric (450 nm) product proportional to the enzymatic activity present. The assay is sensitive to 100 mUnits/mL. One unit of GAPDH activity is the amount of enzyme that will generate 1.0 mmole of NADH per minute at pH 7.2 at 37 °C.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Aquatic Chronic 3 - Eye Dam. 1 - Skin Corr. 1B
Código de classe de armazenamento
8A - Combustible corrosive hazardous materials
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