MAK174
Fatty Acid Extraction Kit, Low Standard
sufficient for 40 extractions
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About This Item
Código UNSPSC:
12161503
NACRES:
NA.25
Produtos recomendados
uso
sufficient for 40 extractions
características do produto alternativo mais ecológico
Waste Prevention
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
aplicação(ões)
cosmetics
food and beverages
sample preservation
categoria alternativa mais ecológica
, Aligned
doença(s) relevante(s)
cancer
temperatura de armazenamento
room temp
Descrição geral
Lipids are structural components of cell membranes that play a critical role in gene transcription, signaling, and metabolism. Several lipid species exist in biological systems, including phospholipids, triglycerides, and fatty acids. When extracting fatty acids at low or very low levels, such as in cellular matrices, use the MAK174 fatty acid extraction kit. When extracting fatty acids at normal or high levels, such as in food matrices, use the MAK338 fatty acid extraction kit.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
The Folch method has been conventionally used to extract lipids containing fatty acids from biological samples, using chloroform, methanol, and water to separate lipids from aqueous-soluble compounds. In this procedure, lipids are retained in the lower chloroform layer; whereas, aqueous-soluble compounds are retained in the upper methanol-water layer. The sample is then centrifuged to achieve uniform separation and the bottom chloroform layer is transferred with a pipette to a new test tube.
Aplicação
Fatty Acid Extraction Kit has been used for fatty acid extraction.
Adequação
This kit is suitable for the extraction of lipids from a variety of samples such as whole blood, serum, biological tissue, food samples, and oil.
Princípio
The Fatty Acid Extraction Kit shortens this extraction process by eliminating the need to prepare solvents and standards, centrifugation, and pipetting. Once the sample is homogenized and dissolved in the Extraction Solvent supplied in our kit, it is inverted twice and poured into the syringe containing a filter, which preferentially elutes the chloroform layer containing total lipids. A portion of the total lipid extract containing fatty acids can then be transesterified for GC-FID analysis.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product reduces solvent waste over traditional methods. Find details here.
Nota de análise
Case study can be found here: Experts have tested the Fatty Acid Extraction Kit: Time savings and greener alternative
Outras notas
Experts have tested the Fatty Acid Extraction Kit. Watch the Video to learn more and to see how the technology works.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 2 - Eye Irrit. 2 - Flam. Liq. 2 - Repr. 2 - Skin Irrit. 2 - STOT RE 1 - STOT SE 1 - STOT SE 3
Órgãos-alvo
Central nervous system, Eyes, Liver,Kidney
Código de classe de armazenamento
3 - Flammable liquids
Ponto de fulgor (°F)
49.5 °F - closed cup
Ponto de fulgor (°C)
9.7 °C - closed cup
Certificados de análise (COA)
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Journal of food science, 82(10), 2291-2297 (2017-08-24)
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