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Documentos Principais

M8662

Sigma-Aldrich

Óleo mineral

PCR Reagent

Sinônimo(s):

Mineral oil for PCR

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About This Item

Número CAS:
Número CE:
Número MDL:
Código UNSPSC:
12181504
NACRES:
NA.52

grau

PCR Reagent

Nível de qualidade

forma

liquid

embalagem

vial of 1.5 mL (Total volume 7.5 mL (5 vials))

técnica(s)

PCR: suitable

cor

colorless

índice de refração

n20/D 1.467 (lit.)

densidade

0.84 g/mL at 25 °C (lit.)

atividade externa

DNase, RNase, protease, none detected

temperatura de armazenamento

room temp

chave InChI

AEOVEGJBKQQFOP-DDVLFWKVSA-L

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

Mineral oil is used as a reaction mix overlay in polymerase chain reactions (PCR) to prevent reaction solutions from evaporating during the thermal cycling in PCR instruments without heated lids.

Aplicação

Mineral oil has been used:
  • for routine PCR amplifications
  • to improve the heat conductivity between the device and the cycler during reverse transcription-polymerase chain reaction (RT–PCR) amplification
  • to prevent evaporation during single-cell MATQ-sequencing performed on Bravo automated liquid handling platform

Características e benefícios

  • Provided in a convenient 5 × 1.5 mL (1 vial) pack size
  • Tested for the absence of DNase, RNase, and protease.

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available

Equipamento de proteção individual

Eyeshields, Gloves


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Protocolos

Method for bacterial genome analysis and detection of pathogens. Minimize false positive PCRs through lab design and reagents tested for use in bacterial PCR applications.

Protocol using antibody mediated hot start polymerase. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Protocol using antibody mediated hot start polymerase with a red dye for easy gel loading. Method has short activation period (<1min), and results in higher yields and more specificity over standard PCR methods.

Hot Start Taq Polymerase protocol to reduce non-specific amplification, with MgCl2 Optimization

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