M1320

Anti-MTA1 antibody, Mouse monoclonal

clone MTA1-213, purified from hybridoma cell culture

• Sinônimo(s): Anti-Metastasis-associated Gene 1, Anti-NuRD-70
• Número MDL: MFCD07370404
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• conjugado: unconjugated
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: MTA1-213, monoclonal
• forma: buffered aqueous solution
• peso molecular: 75-80 kDa
• reatividade de espécies: mouse, human, canine, rat
• concentração: ~2 mg/mL
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1-2 μg/mL using HeLa cell nuclear extract
• Isotipo: IgG1
• nº de adesão UniProt: Q13330
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... MTA1(9112) ; mouse ... Mta1(116870) ; rat ... Mta1(64520)

Descrição

Descrição geral

Monoclonal Anti-MTA1 (mouse IgG1 isotype) is derived from the hybridoma MTA1-213 produced by the fusion of mouse myeloma cells (NS1 cells) and splenocytes from BALB/c mice immunized with a synthetic peptide of human MTA1. Metastasis-associated genes (MTAs) comprise a novel gene family with a growing number of members. Currently, there are three known genes encoding for six isoforms, MTA1, MTA1S, MTA-ZG29p, MTA2/MTA1L1, MTA3, and MTA3L. MTA1, also known as NuRD-70, was originally identified in rat metastatic adenocarcinomas as a differentially expressed gene. It encodes a 715 amino acid protein that shares about 70% overall homology to human MTA2 and MTA3 proteins, the C-terminus being more divergent than the N-terminus.

Imunogênio

synthetic peptide corresponding to amino acids 626-641 of human MTA1.

Aplicação

Monoclonal Anti-MTA1 antibody produced in mouse has been used in enzyme linked immunosorbent assay (ELISA), immunoblotting, immunoprecipitation and immunocytochemistry.

Ações bioquímicas/fisiológicas

Metastasis associated gene 1 (MTA1) is associated with cancer metastasis. MTA1 interacts with the deacetylases within the nuclear remodeling and deacetylation complexes Mi2/ nucleosome remodelling and histone deacetylation (NuRD) suggesting that it is involved in transcriptional repression. MTA1 interacts with cyclin-dependent kinase-activating kinase (CAK), a component of the transcription factor (TFIIH) regulatory complex and act as a signal transducer to mediate crosstalk between corepressor complexes and the general transcription machinery. MTA1 transcriptionally represses estrogen receptor (ER), having serious implications for the development of an aggressive breast cancer phenotype. MTA1s localizes in the cytoplasm and sequesters ER in the cytoplasm, preventing ligand-induced translocation of ER and stimulating malignant phenotypes.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): nwg
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable