H7290

Anti-HtrA2 antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• Sinônimo(s): Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase 2, Anti-Omi, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: rabbit
• conjugado: unconjugated
• forma do anticorpo: affinity isolated antibody
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• Formulário: buffered aqueous solution
• peso molecular: antigen ~36 kDa (also 50 kDa band corresponding to HtrA2 precursor)
• reatividade de espécies: human, rat
• embalagem: antibody small pack of 25 μL
• validação aprimorada: recombinant expression; Learn more about Antibody Enhanced Validation
• concentração: ~1.5 mg/mL
• técnica(s): western blot: 2-4 μg/mL using rat liver microsomal fraction and HEK-293 cells expressing human HTRA2
• nº de adesão UniProt: O43464
• Condições de expedição: dry ice
• temperatura de armazenamento: −20°C
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... HTRA2(27429) ; rat ... Htra2(297376)

Descrição

Descrição geral

High-temperature requirement protein A2 (HtrA2) belongs to the HtrA family of serine proteases. This protein is located in the mitochondria and is made up of N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM), a single C-terminal PDZ domain that mediates protein-protein interaction, and a conserved catalytic domain of serine proteases. The HTRA2 gene is located on the human chromosome at 2p13.1.

Especificidade

Anti-HtrA2 specifically recognizes human and rat HtrA2.

Aplicação

Anti-HtrA2 antibody produced in rabbit may be used in immunoblotting.

Ações bioquímicas/fisiológicas

High-temperature requirement A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. This protein possesses protease activity, which is required for mitochondrial homeostasis. HtrA2 is released from the mitochondria as a mature form in response to apoptotic stimuli and is involved in regulating apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2 promotes cytochrome c-dependent caspase activation by neutralizing inhibitors of apoptosis proteins (IAPs). Mutations that inactivate HtrA2 are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease.

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Armazenamento e estabilidade

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

Exoneração de responsabilidade

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)