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Key Documents

GE28-9644-45

Sigma-Aldrich

HiScale 50/20

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About This Item

Código UNSPSC:
12352200
NACRES:
SB.52

técnica(s)

LPLC: suitable

Categorias relacionadas

Descrição geral

HiScale columns are designed to withstand pressures of up to 20 bar (290 psi) and high flow rates, making them compatible with modern BioProcess chromatography media such as MabSelect and Capto. The user-friendly design allows for an optimized process, through simplified and reproducible operation.
The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments as well as disassembly and cleaning. The measurement scale on the column makes it easier to predict and reach a predefined packing height, thereby increasing process control as well as reproducibility. Although optimized for high-pressure media, HiScale columns are fully compatible with all applications (and packing methods) currently applicable to XK columns.

Características e benefícios

Adapter QuickLock mechanism, column measurement scale, and ergonomic design, facilitate column handling and increase process control.
Based on the well-established line of XK columns and compatible with all methods currently applicable to XK columns.
Capability to rinse entire adapter shaft after packing, thereby minimizing risk of bacterial growth after prolonged use.
Dual adapters, offering high flexibility and dynamic packing heights.
Empty columns with high pressure stability for packing with modern BioProcess media for process development and preparative chromatography.
Provides axial compression of the gel bed and a nonrotating plunger mechanism that expands packing capabilities.

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Artigos

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Protocolos

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

This page describes efficient column packing and preparation for affinity chromatography of GST-tagged proteins using GE Healthcare products.

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from GE Healthcare.

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