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Key Documents

GE17-5072-01

Q Sepharose Xl

Cytiva 17-5072-01, pack of 300 mL

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About This Item

Código UNSPSC:
41106500
NACRES:
NA.56

ligand

quaternary amine

descrição

Ion Exchanger Type (value)

embalagem

pack of 300 mL

fabricante/nome comercial

Cytiva 17-5072-01

matriz

6% cross-linked agarose with dextran surface extenders

tamanho de partícula

45-165 μm

diâmetro médio

90 μm

cleaning in place

2-14

working range

2-12

adequação

suitable for bioprocess medium

Descrição geral

Q Sepharose XL is a strong anion exchanger designed for use in the capture steps of biomolecules from clarified feed-stocks.

Q Sepharose XL is based on a highly cross-linked, bead formed 6% agarose matrix similar to the well established Q Sepharose Fast Flow media. Dextran chains are covalently coupled to the agarose matrix and modified with quaternary ammonium (Q) strong anion exchange groups through chemically stable ether bonds. The stable agarose matrix and long, flexible chains of dextran with bound charged groups work together to increase loading capacity whilst allowing high flow rates.

With its high loading capacities and excellent physical and chemical stability, Q Sepharose XL is suitable for the capture stage of a downstream process, which is characterised by large loads of crude samples.

As member of the BioProcess media range, Q Sepharose XL meets industrial demands with security of supply and comprehensive technical and regulatory support.

Características e benefícios

  • High loading capacities give more productive capture from clarified feedstocks.
  • Strong anion exchanger designed for capture steps
  • High chemical stability for effective CIP/sanitization
  • The hydrophilic nature of the base matrix ensures low levels of non-specific binding leading to low levels of host cell-derived impurities in the elution pool.

Armazenamento e estabilidade

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Nota de análise

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Informações legais

Sepharose is a trademark of Cytiva

Pictogramas

Flame

Palavra indicadora

Warning

Frases de perigo

Código de classe de armazenamento

3 - Flammable liquids


Certificados de análise (COA)

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Artigos

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

This page covers the standard ÄKTAdesign configurations for simple IEX chromatography.

This page shows volatile and non-volatile buffer suggestions for anion and cation exchange chromatography.

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Protocolos

This page covers how and when to use Sepharose XL media for purification of proteins.

This page discusses how to do a purification with SOURCE™ media from GE Healthcare

This page shows how to perform column packing and preparation for ion exchange chromatography and chromatafocusing when using Tricorn or XK columns available from GE Healthcare.

Sample Preparation in Ion Exchange Chromatography

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