GE17-1179-01
Resource™ Q
Cytiva 17-1179-01, pack of 6 mL
Sinônimo(s):
Resource Q Column
About This Item
Produtos recomendados
ligand
quaternary ammonium
descrição
Ion Exchanger Type (value)
embalagem
pack of 6 mL
fabricante/nome comercial
Cytiva 17-1179-01
Parâmetros
87 psi
tamanho do leito
16 mm × 30 mm
volume do leito
6 mL
Matriz
polystyrene/divinylbenzene
Tamanho médio da partícula
15 μm
tamanho de partícula
15-15 μm
cleaning
1-14
Faixa de trabalho
2-12
adequação
suitable for bioprocess medium
Categorias relacionadas
Descrição geral
The small (15 μm) monodisperse bead give high-resolution purification at high flow rates. In addition, hydrophilization of the beads minimizes nonspecific adsorption and allows high recovery of purified sample. The material of the column body is PEEK (polyetheretherketone). SOURCE™ 30Q and 30S media with a 30 μm bead size are designed for intermediate purification and large-scale polishing and allow higher flow rates.
Aplicação
This application note describes a convenient and simple protocol for the purification of synthetic phosporothioate oligonucleotides, using ion exchange chromatography on an automated AKTAexplorer 100 system. These data show that AKTAexplorer 100, controlled by UNICORN, is a convenient way of obtaining reliable and reproducible results, which can also be scaled-up.
Características e benefícios
- SOURCE™ 15Q and 15S media are highly suitable for fast, high-resolution purifications and easy scale-up.
- Rigid, monodispersed, spherical particles with controlled pore-size distribution offer excellent flow characteristics, sample loading of up to 25 mg of protein/mL, and improved stability in organic solvents and pH extremes
- RESOURCE™ prepacked columns for fast separation. Separation times using 1 mL RESOURCE™ columns are less than 3 min at 9.6 mL/min and about 20 min using a peristaltic pump at 1 mL/min.
- Improved capacity compared to MonoBeads but with a slightly lower resolution.
Armazenamento e estabilidade
Nota de análise
Informações legais
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Código de classe de armazenamento
3 - Flammable liquids
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Protocolos
This page covers using SOURCE™ medias for the purification of proteins, peptides, or oligonucleotides.
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This page discusses how to do a purification with SOURCE™ media from GE Healthcare
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