FG0100
Kits e reagentes de triglicerídeos e glicerol livre
1 kit sufficient for 1000 reactions
Sinônimo(s):
Triglyceride and Free Glycerol Kits and Reagents
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About This Item
Código UNSPSC:
12352202
NACRES:
NA.26
Produtos recomendados
Nível de qualidade
uso
kit sufficient for 1000 reactions
método de detecção
colorimetric
temperatura de armazenamento
2-8°C
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Descrição geral
Glycerol, also referred to as glycerin or glycerine, is a 3 carbon sugar alcohol that forms the backbone of fatty acids. It is a central component for synthesis of all lipids, and acts as a backbone for triglycerides and phospholipids, which play an important role in cell membrane structure. Due to its low toxicity, glycerol is widely used in pharmaceutical, food and cosmetic formulations and is the main waste by-product of biodiesel production via transesterification. In addition to kits, the individual reagents and glycerol standard are available separately when fewer reactions are needed.
Aplicação
Os kits de triglicerídeos e glicerol livre destinam-se à determinação quantitativa de glicerol, triglicerídeos totais ou triglicerídeos livres.
The Free Glycerol Determination Kit has been used to determine the level of glycerol released into the medium from mammalian cells grown in vitro .
The Free Glycerol Determination Kit measures free, endogenous glycerol using coupled enzyme reactions and does not include initial lipase hydrolysis. Glycerol is phosphorylated by adenosine-5′-triphosphate (ATP) forming glycerol-1-phosphate (G-1-P) and adenosine-5′-diphosphate (ADP) in the reaction catalyzed by glycerol kinase (GK). G-1-P is then oxidized by glycerol phosphate oxidase (GPO) to dihydroxyacetone phosphate (DAP) and hydrogen peroxide (H2O2). Peroxidase catalyzes the coupling of H2O2 with 4-aminoantipyrine (4-AAP) and sodium N-ethyl-N-(3-sulfopropyl) m-anisidine (ESPA) to produce a quinoneimine dye that shows an absorbance maximum at 540 nm. The increase in absorbance at 540 nm is directly proportional to the free glycerol concentration of the sample.
Adequação
Suitable for the quantitative enzymatic determination of glycerol in serum or plasma.
Ligação
Além dos kits, os reagentes individuais e o padrão de glicerol estão disponíveis separadamente, quando menos reações são necessárias.
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2
Código de classe de armazenamento
10 - Combustible liquids
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