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F8264

Sigma-Aldrich

Anti-Mouse IgG (γ-chain specific)−FITC antibody produced in goat

affinity isolated antibody, buffered aqueous solution

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

fonte biológica

goat

Nível de qualidade

conjugado

FITC conjugate

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

forma

buffered aqueous solution

técnica(s)

direct immunofluorescence: 1:64

temperatura de armazenamento

2-8°C

modificação pós-traducional do alvo

unmodified

Descrição geral

IgG antibody subtype is the most abundant serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defence of the neonate against infections. Anti-Mouse IgG (γ-chain specific)-FITC antibody is specific for is specific for mouse IgG when tested against purified mouse IgA, IgG (all subclasses), and mouse IgM. Goat anti-mouse IgG is isolated by affinity isolation and conjugated to Fluorescein Isothiocyanate (FITC), isomer I.

Imunogênio

Purified mouse IgG

Aplicação

Anti-Mouse IgG (γ-chain specific)-FITC antibody may be used for immunofluorescence of mouse spleen cells at a working antibody dilution of 1:64. For ELISA using mice sera, antibody dilution of 1:50 was used. The antibody was also used in anti-nucear antibody detection using mouse sera and for labeling Saimiri brain endothelial cells for exhaustive photon reassignment microscopy
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Lucia Terlecki-Zaniewicz et al.
Aging, 10(5), 1103-1132 (2018-05-21)
Loss of functionality during aging of cells and organisms is caused and accompanied by altered cell-to-cell communication and signalling. One factor thereby is the chronic accumulation of senescent cells and the concomitant senescence-associated secretory phenotype (SASP) that contributes to microenvironment
C M Shih et al.
Journal of clinical microbiology, 33(12), 3164-3168 (1995-12-01)
We determined whether the infectivity of the Lyme disease spirochete (Borrelia burgdorferi) to vector ticks varies with the duration of infection in laboratory mice. Thus, noninfected nymphal deer ticks were permitted to feed on two strains of early (2 months
Chi G Weindel et al.
Journal of immunology (Baltimore, Md. : 1950), 198(3), 1081-1092 (2016-12-30)
Individuals suffering from autoimmune disorders possess a hyperactive cellular phenotype where tolerance to self-antigens is lost. Autophagy has been implicated in both the induction and prevention of autoimmunity, and modulators of this cellular recycling process hold high potential for the
Monique H M Melis et al.
Oncotarget, 8(55), 93867-93877 (2017-12-08)
Increasing evidence from epidemiological and pathological studies suggests a role of the immune system in the initiation and progression of multiple cancers, including prostate cancer. Reports on the contribution of the adaptive immune system are contradictive, since both suppression and
B Pouvelle et al.
Molecular medicine (Cambridge, Mass.), 3(8), 508-518 (1997-08-01)
Chondroitin-4-sulfate (CSA) was recently described as a Plasmodium falciparum cytoadherence receptor present on Saimiri brain microvascular and human lung endothelial cells. To specifically study chondroitin-4-sulfate-mediated cytoadherence, a parasite population was selected through panning of the Palo-Alto (FUP) 1 P. falciparum

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