Pular para o conteúdo
Merck
Todas as fotos(3)

Key Documents

View All Documentation

E7637

Sigma-Aldrich

Ethidium bromide

BioReagent, for molecular biology, powder

Sinônimo(s):

3,8-Diamino-5-ethyl-6-phenylphenanthridinium bromide, EtBr, Homidium bromide

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(3)

About This Item

Fórmula empírica (Notação de Hill):
C21H20BrN3
Número CAS:
1239-45-8
Peso molecular:
394.31
Beilstein:
3642536
Número CE:
214-984-6
Número MDL:
MFCD00011724
Código UNSPSC:
41105322
ID de substância PubChem:
24894653
NACRES:
NA.31

grau

for molecular biology

Nível de qualidade

200

linha de produto

BioReagent

forma

powder

técnica(s)

electrophoresis: suitable

pf

260-262 °C (dec.) (lit.)

fluorescência

λex 360 nm; λem 590 nm in PBS
λex 526 nm; λem 605 nm (10 mM TBE; pH 8.0;)
λex 526 nm; λem 605 nm in aqueous buffer

adequação

suitable for gel electrophoresis

cadeia de caracteres SMILES

[Br-].CC[n+]1c(-c2ccccc2)c3cc(N)ccc3c4ccc(N)cc14

InChI

1S/C21H19N3.BrH/c1-2-24-20-13-16(23)9-11-18(20)17-10-8-15(22)12-19(17)21(24)14-6-4-3-5-7-14;/h3-13,23H,2,22H2,1H3;1H

chave InChI

ZMMJGEGLRURXTF-UHFFFAOYSA-N

Procurando produtos similares? Visita Guia de comparação de produtos

Aplicação

Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase.

Frameshift mutagen which intercalates double-stranded DNA and RNA.

Ações bioquímicas/fisiológicas

Ethidium bromide intercalates double-stranded DNA and RNA and acts as a frameshift mutagen. It can also be used in conjunction with acridine orange to differentiate between viable, apoptotic and necrotic cells.

Reconstituição

For staining a gel after electrophoresis, dilute a sample of the stock solution to 0.5 μg/ml with water and incubate the gel for 15-30 min. Destaining is usually not needed but can be carried out in water for 15 min if decreased background is necessary. The DNA bands can then be detected on a UV light box (254 nm wavelength). Ethidium bromide can also be incorporated into the gel and running buffer at 0.5 μg/ml and visualized immediately after electrophoresis.

produto relacionado

Nº do produto
Descrição
Preços

Pictogramas

Skull and crossbonesHealth hazard
GHS06,GHS08

Palavra indicadora

Danger

Frases de perigo

H302,H330,H341

Classificações de perigo

Acute Tox. 1 Inhalation - Acute Tox. 4 Oral - Muta. 2

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Anna Mas-Vinyals et al.
Colloids and surfaces. B, Biointerfaces, 181, 864-871 (2019-08-07)
Regenerative medicine requires the use of heterogeneous scaffolds when the tissue that needs to be repaired presents a gradient in its properties and cannot be replaced by a homogeneous graft. Then, an intimate contact between the different layers is critical
Ulrich Braunschweig et al.
Genome research, 24(11), 1774-1786 (2014-09-27)
Alternative splicing (AS) of precursor RNAs is responsible for greatly expanding the regulatory and functional capacity of eukaryotic genomes. Of the different classes of AS, intron retention (IR) is the least well understood. In plants and unicellular eukaryotes, IR is
Patrick Seitz et al.
PLoS genetics, 10(1), e1004066-e1004066 (2014-01-07)
The DNA uptake of naturally competent bacteria has been attributed to the action of DNA uptake machineries resembling type IV pilus complexes. However, the protein(s) for pulling the DNA across the outer membrane of Gram-negative bacteria remain speculative. Here we
Li Zhang et al.
Comparative biochemistry and physiology. Part A, Molecular & integrative physiology, 186, 39-51 (2014-12-04)
Bill Milsom has made seminal contributions to our understanding of ventilatory control in a wide range of vertebrates. Teleosts are particularly interesting, because they produce a 3rd, potentially toxic respiratory gas (ammonia) in large amounts. Fish are well known to
Yuanhao Zhang et al.
PloS one, 10(6), e0130902-e0130902 (2015-07-01)
Adherent-invasive Escherichia coli (AIEC) strains are detected more frequently within mucosal lesions of patients with Crohn's disease (CD). The AIEC phenotype consists of adherence and invasion of intestinal epithelial cells and survival within macrophages of these bacteria in vitro. Our
Exibir todos os documentos relacionados

Conteúdo relacionado

Ethidium Bromide

Ethidium bromide is a well-known and widely used fluorescent dye in biotechnology research.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica