CS0030
Senescence Cells Histochemical Staining Kit
sufficient for 100 tests
Sinônimo(s):
Senescent cells IHC kit, cellular β-galactosidase probe
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About This Item
Código UNSPSC:
12352207
NACRES:
NA.32
Produtos recomendados
uso
sufficient for 100 tests
Nível de qualidade
embalagem
pkg of 1 kit
condição de armazenamento
dry at room temperature
método de detecção
colorimetric
Condições de expedição
dry ice
temperatura de armazenamento
−20°C
Descrição geral
The Senescence Cells Histochemical Staining Kit contains all the reagents required for identifying senescent cells using an assay based on a histochemical stain for β-galactosidase activity at pH 6. β-galactosidase activity is detectable in senescent cells, but not in quiescent, immortal, or tumor cells. Cellular senescence is a progression of events, whereby cells move from an actively dividing to a non-dividing stage. The cellular senescence process is associated with aging. The decrease in cell division is virtually irreversible and complete. In conjunction with the loss of the ability to divide, changes occur in the morphology, shape, and physical appearance of the cells, and their pattern of gene expression. At the end of the process cell death usually occurs, although the cells may remain viable for a long time.
Aplicação
Senescence Cells Histochemical Staining Kit has been used for:
- senescence-associated β-galactosidase assay
- to test for senescence in human pancreatic cancer cells
- adipose-derived stem cells (ADSCs)
- cord blood mesenchymal stromal/stem cells (CBMSC)
Ações bioquímicas/fisiológicas
Replicative senescence is a growth-arrest state associated with loss of division potential, changes in cell morphology, shape and physical appearance, and the pattern of gene expression in cells.
Informações legais
Manufactured under license to US Patent Nos. 5,491,069 and 5,795,728.
Somente componentes do kit
Nº do produto
Descrição
- X-gal solution 4 mL
- Staining Solution, 10X 15 mL
- Fixation Buffer 10x 15 mL
- Reagent B 1.5 mL
- Reagent C 1.5 mL
- Dulbecco's Phosphate Buffered Saline (PBS) 10x 60 mL
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Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Acute Tox. 3 Inhalation - Acute Tox. 4 Dermal - Acute Tox. 4 Oral - Aquatic Chronic 3 - Carc. 1B - Eye Dam. 1 - Muta. 2 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
Órgãos-alvo
Respiratory system
Perigos de suplementos
Código de classe de armazenamento
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
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G P Dimri et al.
Proceedings of the National Academy of Sciences of the United States of America, 92(20), 9363-9367 (1995-09-26)
Normal somatic cells invariably enter a state of irreversibly arrested growth and altered function after a finite number of divisions. This process, termed replicative senescence, is thought to be a tumor-suppressive mechanism and an underlying cause of aging. There is
B van der Loo et al.
Experimental cell research, 241(2), 309-315 (1998-06-25)
A beta-galactosidase activity has recently been used as a histochemical marker of replicative senescence in human fibroblasts and keratinocytes. To establish whether this marker could be used to detect senescence of vascular cells, we have investigated its presence in cultures
Mandy Oj Grootaert et al.
Autophagy, 11(11), 2014-2032 (2015-09-24)
Autophagy is triggered in vascular smooth muscle cells (VSMCs) of diseased arterial vessels. However, the role of VSMC autophagy in cardiovascular disease is poorly understood. Therefore, we investigated the effect of defective autophagy on VSMC survival and phenotype and its
Natalja E Fedorovich et al.
Biomaterials, 30(3), 344-353 (2008-10-22)
Photopolymerizable hydrogels, formed by UV-exposure of photosensitive polymers in the presence of photoinitiators, are widely used materials in tissue engineering research employed for cellular entrapment and patterning. During photopolymerization, the entrapped cells are directly exposed to polymer and photoinitiator molecules.
Hadis Shakeri et al.
Cardiovascular research, 114(7), 1041-1051 (2018-03-13)
Cardiovascular ageing is a key determinant of life expectancy. Cellular senescence, a state of irreversible cell cycle arrest, is an important contributor to ageing due to the accumulation of damaged cells. Targeting cellular senescence could prevent age-related cardiovascular diseases. In
Artigos
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
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