GM1899A

98120701, human unknown/unspecified, Lymphoblastoid

• Código UNSPSC: 41106514

Propriedades

• product name: GM1899A, 98120701
• fonte biológica: human unknown/unspecified
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human lymphoblast, HPRT - deficient

Descrição de linhagem celular

The lymphoblastoid cell line GM1899A has been derived from a Lesch-Nyhan patient. It is deficient for hypoxanthine-guanine phosphoribosyl transferase (HPRT) with a point mutation in the respective gene. This cell line is used as a feeder for extended-term culture of human T-lymphocytes (see LB lines in the collection).

Aplicação

Feeder for T-lymphocyte culture

Meio de cultura

Dutch Modified RPMI 1640 + 2mM Glutamine + 20mM Hepes + 2mM Sodium Pyruvate (NaPy) + 20% Heated Inactivated Foetal Bovine Serum (HI-FBS)

Rotina de subcultura

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO₂; 37°C. Do not dilute below 1 x 100,000 cells/ml, wash 2-3 times after resuscitation before seeding; disaggregate every few days by pipetting to avoid formation of large aggregates

Outras notas

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