MOR/0.4R

96042334, human lung, Not specified

• Código UNSPSC: 41106514
• fonte biológica: human lung
• modo de crescimento: Semi-adherent aggregates
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• doença(s) relevante(s): cancer

Propriedades

• Nome do produto: MOR/0.4R, 96042334
• fonte biológica: human lung
• modo de crescimento: Semi-adherent aggregates
• cariótipo: Not specified
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human lung adenocarcinoma, drug-resistant

Descrição de linhagem celular

MOR/0.4R has been developed from the parent line, MOR, by continuous exposure to increasing concentrations of doxorubicin (also known as adriamycin). This drug-resistant cell line accumulates lower levels of doxorubicin than the parent line and have been shown to overexpress multi drug resistance-associated protein (MRP). The expression of a 190kDa membrane protein associated with the degree of drug resistance has been indicated. Cells grow as easily detaching aggregates

Aplicação

Analysis of acquired drug resistance, MRP studies

Perfil do DNA

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 12
D16S539: 9
D5S818: 12
D7S820: 9
THO1: 7
TPOX: 11
vWA: 15

Meio de cultura

RPMI 1640 + 2mM Glutamine + 0.4μg/ml Doxorubicin + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split subconfluent cultures (70-80%) 1:2 to 1:4 seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. It is recommended to culture the cells without drug after resuscitation until the first passage. These cells produce a

Outras notas

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