MCP21

96030418, Reactivity: Anti-Proteasomes, human

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Mouse x Mouse Hybridoma
• ImmunoDon: BALB/c mouse spleen
• Mieloma: Sp2/0
• Reatividade: Anti-Proteasomes, human
• técnica(s): cell culture | mammalian: suitable
• Isotipo: IgG1
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Mouse Sp2/0 x Mouse BALB/c spleen

Descrição de linhagem celular

Antibodies secreted by the hybridoma line MCP21 react with subunit C3 in human and rabbit proteasomes, both with intact proteasomes and on blots. The hybridoma has been produced by repeated intraperitoneal immunisation of BALB/c mice with 20-50 μg of human placenta proteasomes and subsequent fusion of spleen cells with myeloma Sp2/0.

Imunogênio

Human placenta proteasomes

Aplicação

Detection and purification of proteasomes and analysis of subunits

Meio de cultura

DMEM + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Maintain cultures between 5x10,000 and 1x1,000,000 cells/ml; 5% CO₂; 37°C

Outras notas

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