KYSE-70

94072012, human esophagus, Epithelial

• Sinônimo(s): KY70 Cells, KYSE70 Cells
• Código UNSPSC: 41106514

Propriedades

• product name: KYSE-70, 94072012
• fonte biológica: human esophagus
• modo de crescimento: Adherent
• cariótipo: Hypotriploid with 8% polyploidy
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human oesophageal squamous cell carcinoma

Descrição de linhagem celular

Established from a poorly differentiated invasive oesophageal squamous cell carcinoma resected from middle intra-thoracic oesophagus of a 77-year-old Japanese man prior to treatment. This cell line has been mycoplasma eradicated at ECACC. By short tandem repeat (STR)-PCR analysis the Y chromosome could not be detected in this cell line. It is a known phenomenon that SSC cell lines can lose their Y chromosome.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rotina de subcultura

Split confluent cultures 1:5 to 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Doubling time ~35 hours.

Outras notas

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