H69

91091802, human lung, Not specified

• Sinônimo(s): H69 Cell Line
• Código UNSPSC: 41106514

Propriedades

• product name: H69, 91091802
• fonte biológica: human lung
• modo de crescimento: Aggregates in suspension
• cariótipo: Aneuploid
• morfologia: Not specified
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): metastasis
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian lung small cell carcinoma

Descrição de linhagem celular

This cell line has been established from the pleural fluid of a 55 year old Caucasian male with small cell carcinoma of the lung prior to treatment. Inoculated into athymic mice, the cells form tumours with a typical histology of the original biopsy specimen. Elevated levels of the biomarkers L-dopa decarboxylase, bombesin-like immunoreactivity, neuron-specific enolase and brain isozyme of creatine kinase were reported. Cells grow as spherical floating aggregates.

Aplicação

Tumourigenicity studies

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 12
D16S539: 11
D5S818: 11,13
D7S820: 9
THO1: 8,9
TPOX: 10
vWA: 16,17

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 6x10,000 to 3x100,000 cells/ml; 5% CO₂; 37°C.

Outras notas

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