SCC-4

89062002, human tongue, squamous cell carcinoma. Epithelial morphology.

• Código UNSPSC: 41106514

Propriedades

• product name: SCC-4, 89062002
• fonte biológica: human mouth
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Epidermal keratins; 40 kD Keratin
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human tongue squamous carcinoma

Descrição de linhagem celular

Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.

Meio de cultura

DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone

Rotina de subcultura

Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C

Outras notas

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