C-4 II

88102701

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human cervix
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian cervical carcinoma

Descrição de linhagem celular

From a cervical carcinoma of a 41 year old Caucasian female. Cells form spreading predominantly monolayered, epithelial colonies with hemicysts which are characteristic of transporting epithelia and respond to crowding by separation and shedding. These cells have retained properties of basal cell differentiation in long term culture and exhibit G6PD type B. C-4 II cells have been reported to contain human papilloma virus 18 (HPV-18) DNA sequences and to express HPV-18 RNA.

Aplicação

Differentiation studies

Meio de cultura

Waymouth′s MB 752/1 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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