MRC-5 SV1 TG1

85042501, human lung (fetal), Fibroblast

• Código UNSPSC: 41106514

Propriedades

• product name: MRC-5 SV1 TG1, 85042501
• fonte biológica: human lung (fetal)
• modo de crescimento: Adherent
• cariótipo: Modal no. = 60-80 hyperdiploid
• morfologia: Fibroblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human foetal lung, SV40 transformed

Descrição de linhagem celular

A clonal derivative of an SV40 transformed permanent line of MRC-5. Selection was made using X-ray induction in the presence of 2μg/ml thioguanine. The cells are HAT sensitive.

Meio de cultura

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 1-2x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

Outras notas

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