OX-115

13082229, from Rat x Rat Hybridoma

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• Mieloma: Y3
• Reatividade: Recombinant CD147 domain-0
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

The hybridoma cell line OX-115 recognises an alternatively spliced domain of mouse CD147.

Imunogênio

Recombinant CD147

Aplicação

The isotype of the monoclonal antibody produced by this cell line is rat IgG2a.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rotina de subcultura

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Outras notas

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.