OX-114

13082227, from Rat x Rat Hybridoma

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: Rat x Rat Hybridoma
• ImmunoDon: Rat DA spleen
• Mieloma: Y3
• Reatividade: Mouse CD147
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Descrição de linhagem celular

The hybridoma cell line OX-114 recognises mouse CD147 secretes monoclonal IgG1 antibodies reactive with rat L-selectin.

Imunogênio

Recombinant CD147

Aplicação

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

Meio de cultura

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

Rotina de subcultura

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

Outras notas

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.