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Key Documents

C-12620

Sigma-Aldrich

Human Nasal Epithelial Cells (HNEpC)

500,000 cryopreserved cells

Sinônimo(s):

HNEpC cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

fonte biológica

human nose (nasal mucosa)

embalagem

pkg of 500,000 cells

morfologia

(epithelial)

técnica(s)

cell culture | mammalian: suitable

Condições de expedição

dry ice

temperatura de armazenamento

−196°C

Descrição geral

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origem de linhagem celular

Nose

Aplicação

Primary Human Nasal Epithelial Cells (HNEpC) are isolated from normal human nasal mucosa and are provided in a cryopreserved format. HNEpC stain positive for cytokeratin. The epithelium in the nasal cavity cleans, humidifies, and warms inhaled air. In addition, it produces mucus, which binds particles that are subsequently transported to the pharynx by cilia on the epithelial cells. HNEpC are useful for in vitro studies of these processes.

Qualidade

Rigid quality control tests are performed for each lot of Epithelial Cells. They are tested for cell morphology, adherence rate and cell viability. Furthermore, flow cytometric analyses for the cell-type specific marker cytokeratin are carried out for each lot. Growth performance is tested through multiple passages under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertência

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rotina de subcultura

Click here for more information.

Outras notas

Recommended Plating Density: 10000 - 15000 cells per cm2Passage After Thawing: P2Tested Markers: Cytokeratin positiveGuaranteed population doublings: >15

Produtos recomendados

Recommended Primary Cell Culture Media:Link

Exoneração de responsabilidade

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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Artigos

Primary human airway epithelial cells and serum-free growth media are optimized for the in vitro cultivation of epithelial cells from large air passages. Protocols for handling, cell passaging, media, and product use.

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