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68221

Sigma-Aldrich

Abberior® STAR 440SXP, NHS ester

for long Stokes STED and 2-color STED application

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About This Item

Código UNSPSC:
12352108
NACRES:
NA.32

Ensaio

≥80.0% (degree of coupling)

forma

solid

peso molecular

Mw 597.5 g/mol

solubilidade

DMF: 1 mg/mL, clear

fluorescência

λex 440 nm; λem 511 nm±10 nm in PBS, pH 7.4

temperatura de armazenamento

−20°C

Descrição geral

Absorption Maximum, λmax: 433 nm (MeOH)
432 nm (PBS, pH 7.4)
Extinction Coefficient, ε(λmax): 36,000 M-1cm-1 (MeOH)
33,000 M-1cm-1 (PBS, pH 7.4)
Correction Factor, CF260 = ε260/εmax: 0.47 (PBS, pH 7.4)
Correction Factor, CF280 = ε280/εmax: 0.31 (PBS, pH 7.4)
Fluorescence Maximum, λfl: 502 nm (MeOH),
511 nm (PBS, pH 7.4)
Recommended STED Wavelength, λSTED: 590 − 620 nm
Fluorescence Quantum Yield, η: 0.57 (PBS, pH 7.4)
Fluorescence Lifetime, τ: 3.7 ns (PBS, pH 7.4)

Aplicação

  • Abberior STAR 440SXP goat anti-mouse antibody has been used for STED (stimulated emission depletion) microscopy imaging of primary hippocampal cells.
  • Secondary anti-mouse antibody conjugated with Abberior STAR 440SXP has been used for gated STED microscopy imaging of viral capsid.
  • Abberior STAR 440SXP goat anti-rabbit antibody has been used for two-color STED microscopy imaging of olfactory epithelium.

Adequação

Designed and tested for fluorescent super-resolution microscopy

Informações legais

6538 is a trademark of American Type Culture Collection
abberior is a registered trademark of Abberior GmbH

produto relacionado

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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I-Hsuan Wang et al.
Cell host & microbe, 14(4), 468-480 (2013-10-22)
Viral DNA trafficking in cells has large impacts on physiology and disease development. Current methods lack the resolution and accuracy to visualize and quantify viral DNA trafficking at single-molecule resolution. We developed a noninvasive protocol for accurate quantification of viral DNA-genome
Linda Westin et al.
BMC neuroscience, 15, 45-45 (2014-03-29)
Norbin is a neuron-specific, cytosolic protein that interacts with the metabotropic glutamate receptor 5 (mGluR5) and has a profound impact on mGluR5 signaling. Yet, little is known about its synaptic distribution. Here we have analyzed the spatial relationship between Norbin
Bastian Henkel et al.
Chemical senses, 40(2), 73-87 (2014-12-17)
Vertebrates can sense and identify a vast array of chemical cues. The molecular machinery involved in chemodetection and transduction is expressed within the cilia of olfactory sensory neurons. Currently, there is only limited information available on the distribution and density
S W Hell et al.
Optics letters, 19(11), 780-782 (1994-06-01)
We propose a new type of scanning fluorescence microscope capable of resolving 35 nm in the far field. We overcome the diffraction resolution limit by employing stimulated emission to inhibit the fluorescence process in the outer regions of the excitation
Marcus Dyba et al.
Nature biotechnology, 21(11), 1303-1304 (2003-10-21)
We report immunofluorescence imaging with a spatial resolution well beyond the diffraction limit. An axial resolution of approximately 50 nm, corresponding to 1/16 of the irradiation wavelength of 793 nm, is achieved by stimulated emission depletion through opposing lenses. We

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