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Sigma-Aldrich

Atto 550-Biotin

BioReagent, suitable for fluorescence, ≥90% (HPLC)

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About This Item

Código UNSPSC:
12352108
eCl@ss:
34058011
NACRES:
NA.32

linha de produto

BioReagent

Nível de qualidade

Ensaio

≥90% (HPLC)

Formulário

film

fabricante/nome comercial

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

Absorção UV

λ: 553-559 nm Amax

adequação

suitable for fluorescence

temperatura de armazenamento

−20°C

Aplicação

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550, which is similar to Cy3, may be useful in applications such as fluorescence resonance energy transfer (FRET) and as a tag for molecules such as secondary antibodies. Atto 550-Biotin is suitable for fluorescence labeling of avidin/streptavidin conjugated molecules.

Informações legais

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

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Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Atto 550

Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
J Oertel et al.
Journal of cancer research and clinical oncology, 126(8), 425-440 (2000-08-29)
Immunocytochemical investigations of fixed cells are used to enhance diagnostic accuracy in haematology and oncology. The alkaline-phosphatase/anti(alkaline phosphatase) technique, immunoperoxidase and the avidin-biotin technique are the most important methods in immunocytochemistry. Tyramide-enhanced immunostaining is a new powerful technique. Peripheral blood
Immunohistochemical methods for the demonstration of tumor markers.
H Denk
Current topics in pathology. Ergebnisse der Pathologie, 77, 47-69 (1987-01-01)
J Chevalier et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 45(4), 481-491 (1997-04-01)
Biotin was recently applied to detect cellular DNA or RNA. In combination with avidin, streptavidin or antibody, it can be conjugated with fluorescent dye, enzyme, ferritin, or gold. However, emphasis has recently been placed on the false-positive results that are
Robert H Meltzer et al.
Lab on a chip, 11(5), 863-873 (2011-01-21)
Rapid, specific, and sensitive detection of airborne bacteria, viruses, and toxins is critical for biodefense, yet the diverse nature of the threats poses a challenge for integrated surveillance, as each class of pathogens typically requires different detection strategies. Here, we

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