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P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Sinônimo(s):

Ponceau S

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About This Item

Fórmula empírica (Notação de Hill):
C22H12N4Na4O13S4
Número CAS:
Peso molecular:
760.57
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

product name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

grau

for protein analysis

Nível de qualidade

linha de produto

BioReagent

forma

liquid

concentração

0.1 % (w/v) in 5% acetic acid

densidade

1.01 g/cm3 at 20 °C

adequação

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

room temp

cadeia de caracteres SMILES

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

chave InChI

KQHKSGRIBYJYFX-WNDMICSESA-J

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Descrição geral

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Aplicação

Ponceau S solution has been used for membrane staining (Western blotting).

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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Artigos

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

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