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Documentos Principais

11218611910

Roche

DNA Molecular Weight Marker VI, DIG-labeled

greener alternative

solution, pkg of 500 μL (10 μg/ml)

Sinônimo(s):

DNA marker

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About This Item

Código UNSPSC:
41105335

Formulário

solution

Nível de qualidade

embalagem

pkg of 500 μL (10 μg/ml)

fabricante/nome comercial

Roche

características do produto alternativo mais ecológico

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoria alternativa mais ecológica

temperatura de armazenamento

−20°C

Descrição geral

DNA Molecular Weight Marker VI, DIG-labeled is a fragment mixture prepared by cleavage of pBR328 DNA and pBR328 DNA with restriction endonucleases Bgl I and Hinf I respectively. The size of the fragment ranges from 0.15 to 2.1 kbp.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical.  The DIG System was established as a sensitive and cost-effective alternative to using radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.

Aplicação

DNA Molecular Weight Marker VI, DIG-labeled, has been used as a size standard in Southern blot analysis for nucleic acid labeling and detection. It has also been used as a size standard for amplified DNA.

Componentes

Ready-to-use solutions in 10mM Tris-HCl, 1mM EDTA, pH 8.0.

Sequência

The mixture contains 12 fragments wit the following base pair lengths: 154/154, 220, 234/234, 298/298, 394, 453, 517, 653, 1033, 1230, 1766, and 2176 bp.
Note: Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.

Outras notas

12 fragments: 154/154; 220; 234/234; 298/298; 394; 453; 517; 653; 1,033; 1,230; 1,766; 2,176 bp
Fragment lengths are derived from computer analysis of the DNA sequence. Depending on the size range of the marker, the smallest fragments will only be visible on overloaded gels.
For life science research only. Not for use in diagnostic procedures.

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

nwg

Ponto de fulgor (°F)

No data available

Ponto de fulgor (°C)

No data available


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Artigos

Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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