Pular para o conteúdo
Merck
Todas as fotos(1)

Key Documents

SCT127

Sigma-Aldrich

BioTracker 640 Red C2(FM4-64) Synaptic Dye

Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.

Sinônimo(s):

Live cell imaging probe

Faça loginpara ver os preços organizacionais e de contrato


About This Item

Código UNSPSC:
12352207
NACRES:
NA.47

técnica(s)

cell based assay: suitable

método de detecção

fluorometric

Condições de expedição

wet ice

Descrição geral

BioTracker synaptic dyes are a series of fluorescent cationic styryl dyes developed to follow synaptic activities at neuromuscular junctions or synapses. These dyes typically have a lipophilic tail at one end and a highly hydrophilic at the other end. In the presence of cells or tissue preparations, the dyes partition between the aqueous phase, where the dyes are virtually non-fluorescent, and the outer leaflet of the cell surface membranes, where the dyes insert the lipophilic end into the membranes and become intensely fluorescent. During endocytosis following nerve stimulation, the dyes become trapped inside the vesicles. Thus, after washing off the dyes on the cell surface, the fluorescent signal is proportional to the number of newly formed vesicles. On the other hand, during exocytosis, the dyes are released from the vesicles along with neurotransmitters, causing a decrease in fluorescent signal. As a result, the change in fluorescent intensity reflects the amount of endocytosis/exocytosis or synaptic activity.

Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm

Aplicação

Live cell fluorescent imaging
Live cell imaging synaptic dye that detects neural synaptic activitiy at neuromuscular junctions and synapses.
Research Category
Cell Imaging
Research Sub Category
Live Cell Dye

Qualidade


Spectral Properties
Absorbance (Membranes): 510 nm
Emission (Membranes): 750 nm

forma física

Lyophilized

Armazenamento e estabilidade

Store BioTracker 640 Red C2(FM4-64) Synaptic Dye at 2-8°C, protected from light. Stock solutions can be prepared at 10 mM and stored at 4°C or -20°C for six months or longer.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica