SCT036
BioTracker 625 Red GSH Dye
Live cell imaging dye for glutathione (GSH) that detects reactive oxygen species (ROS) free radicals.
Sinônimo(s):
Live cell imaging probe
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About This Item
Código UNSPSC:
12352207
eCl@ss:
32161000
NACRES:
NA.47
Produtos recomendados
técnica(s)
cell based assay: suitable
método de detecção
fluorometric
Condições de expedição
ambient
Descrição geral
Glutathione (GSH) is an important antioxidant preventing damage to important cellular components caused by reactive oxygen species (ROS) including free radicals, peroxides, lipid peroxides, and heavy metals. Alterations in glutathione homeostasis are associated with a variety of diseases; therefore, real-time live-cell imaging and quantification of GSH dynamics are important for understanding pathophysiological processes.
The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.
Spectral Properties
Absorbance: 520nm
Emission: 625nm and 582nm
The BioTracker 625 Red GSH Dye is probe for glutathione (GSH) in living cells. The dye is formed with an orange fluorescent dye (T) and a deep-red fluorescent dye (S). At a low GSH concentration, green light excitation of T, leads to an energy transfer to S and a subsequent emission of deep-red fluorescent light. At a high GSH concentration, the energy transfer does not occur, so the probe emits the orange fluorescent light from the emission of T. The dye produces a quick and reversible response to the GSH concentration changes, relatively slow photobleaching and low cytotoxicity.
Spectral Properties
Absorbance: 520nm
Emission: 625nm and 582nm
Aplicação
Live cell fluorescent imaging
Research Category
Cell Imaging
Cell Imaging
Research Sub Category
Live Cell Dye
Live Cell Dye
forma física
Lyophilized
Armazenamento e estabilidade
Store BioTracker 625 Red GSH Dye at -20°C, desiccate and protect from light
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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