SCCAR10148

Assay Ready MB49 Mouse Bladder Carcinoma Cell Line

• Sinônimo(s): MB-49, mouse bladder carcinoma, murine bladder carcinoma
• Código UNSPSC: 41106514
• NACRES: NA.81

Propriedades

• fonte biológica: human
• Nível de qualidade: 100
• embalagem: vial of 1 vial
• fabricante/nome comercial: Millipore
• técnica(s): cell culture | stem cell: suitable
• Condições de expedição: liquid nitrogen
• temperatura de armazenamento: −196°C

Descrição

Descrição geral

MB49 cells are derived from C57BL/Icrf-a′ mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture (1). Transformed cells transplanted into syngeneic mice were shown to generate carcinomas (1). While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed (2). This abnormality is a frequent early event in human bladder cancer.

A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth (3). MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone, human chorionic gonadotrophin (hCG) (3).

MB49 cells exhibit low to no expression of MHC-Class I and II molecules (4). However, upon exposure to IFN-?, the expressions of MHC Class I and II are significantly upregulated (4).

References:
1. Summerhayes IC, Franks LM (1979) Effects of donor age on neoplastic transformation of adult mouse bladder epithelium in vitro. J Natl Cancer Inst 62(4): 1017 - 1023.
2. Fabris VT, Lodilinsky C, Pampena MB, Belgorosky D, Lanari C, Eiján AM (2012) Cytogenetic characterization of the murine bladder cancer model MB49 and the derived invasive line MB49-I. Cancer Genet 205(4): 168 - 76.
3. White-Gilbertson S, Davis M, Voelkel-Johnson C, Kasman LM (2016) Sex differences in the MB49 syngeneic, murine model of bladder cancer. Bladder (San Franc) 3(1): PMID: 26998503.
4. Lattime EC, Gomelia LG, McCue PA (1992) Murine bladder carcinoma cells present antigen to BCG-specific CD4+ T-cells. Cancer Res 52(15): 4286 - 90.

Características e benefícios

MB49 implantation in mice resulted in significantly larger tumors in males than females. In the presence of dihydrotestosterone, MB49 cells exhibited enhanced proliferation in a dose-dependent manner. In contrast, MB49 cells were unresponsive to the pregnancy hormone human chorionic gonadotrophin (hCG) [3]. MB49 cells exhibit low to no expression of MHC-Class I and II molecules [4]. However, upon exposure to IFN-?, the expressions of MHC Class I and II are significantly upregulated

Descrição-alvo

MB49 cells are derived from C57BL/Icrf-a mouse bladder epithelial cells that were transformed by a single 24-hour treatment with the chemical carcinogen 7, 12-dimethylbenz[a]anthracene (DMBA) on the second day of a long term primary culture [1]. Transformed cells transplanted into syngeneic mice were shown to generate carcinomas [1]. While of male origin, karyotype analyses indicate the loss of the Y chromosome in 100% of the cells analyzed [2]. This abnormality is a frequent early event in human bladder cancer. A recent study indicates that MB49 cells recapitulate key features of sex differences in bladder tumor growth.

Armazenamento e estabilidade

MB49 cells should be stored in liquid nitrogen.

Outras notas

This product is intended for sale as a consumable. It may not be propagated, passaged, or expanded and is subject to the terms of the “Assay Ready Cells Research Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@milliporesigma.com.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable