MABS497

Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2

clone 4B7.2, from mouse

• Sinônimo(s): Cytochrome P450 26B1, Cytochrome P450 26A2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450, subfamily XXVIB, polypeptide 1, Cytochrome P450RAI-2, Retinoic acid-metabolizing cytochrome
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 4B7.2, monoclonal
• reatividade de espécies: mouse, rat, human
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG2aκ
• nº de adesão NCBI: NP_063938
• nº de adesão UniProt: Q9NR63
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... CYP26B1(56603)

Descrição

Descrição geral

Cytochrome P450 26B1 (UniProt Q9NR63; also known as Cytochrome P450 26A2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450, subfamily XXVIB, polypeptide 1, Cytochrome P450RAI-2, Retinoic acid-metabolizing cytochrome) is encoded by the CYP26B1 (also known as CYP26A2, P450RAI2, RHFCA) gene (Gene ID 56603) in human. Cytochromes P450 (CYP) proteins are primarily membrane-associated oxidases located either in the inner membrane of mitochondria or in the endoplasmic reticulum. In addition to processing endogenous substrates, CYPs also metabolize exogenous drugs and potentially toxic chemicals. The human CYP superfamily consists of 57 genes and more than 59 pseudogenes divided into 18 families and 43 subfamilies. The CYP26 subfamily of enzymes (CYP26A1, CYP26B1/CYP26A2, CYP26C1) are retinoic acid hydroxylases responsible for the inactivation of all-trans-retinoic acid to hydroxylated forms, such as 4-oxo-, 4-OH-, and 18-OH-all-trans-retinoic acid. CYP26B1 is overexpressed in colorectal cancer (CRC) cells, high CYP26B1 expression level in CRC patients is associated with poor prognosis. CYP26B1 mutations are found in radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA), a disease characterized by craniofacial malformations, occipital encephalocele, radiohumeral fusions, oligodactyly, advanced osseous maturation, and calvarial mineralization defects.

Especificidade

Expected to react with all three spliced isoforms of human CYP26B1 reported by UniProt (Q9NR63).

Imunogênio

Epitope: Internal (C-terminal half).

GST-tagged recombinant internal fragment from the C-terminal half of human Cytochrome P450 26B1.

Aplicação

Anti-Cytochrome P450 26B1 Antibody, clone 4B7.2 is an antibody against Cytochrome P450 26B1 for use in Western Blotting, Immunohistochemistry (Paraffin).

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cytochrome P450 26B1 in human brain, mouse brain, and rat cerebellum tissue.

Research Category
Signaling

Research Sub Category
Toxicology & Drug Resistance

Qualidade

Evaluated by Western Blotting in mouse brain tissue microsomal lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected Cytochrome P450 26B1 in 10 µg of mouse brain tissue microsomal lysate.

Descrição-alvo

~57 kDa observed. 57.51 kDa (isoform 1), 49.01 kDa (isoform 2), and 55.76 kDa (isoform 3) calculated. Uncharacterized band(s) may appear in some lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable