MABS415

Anti-PPM1A, clone 7F12 Antibody

clone 7F12, from mouse

• Sinônimo(s): PP2C-alpha, Protein phosphatase IA, Protein phosphatase 2C isoform alpha, PP2CA, PPM1A, PPPM1A
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 7F12, monoclonal
• reatividade de espécies: human
• técnica(s): ELISA: suitable; flow cytometry: suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão UniProt: P35813
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... PPM1A(5494)

Descrição

Descrição geral

Protein phosphatase 1A, also known as Protein phosphatase 2C isoform alpha (PP2C-alpha) or Protein phosphatase IA, and encoded by the gene name PPM1A, is a member of the PP2C family. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase dephosphorylates, and negatively regulates the activities of, MAP kinases and MAP kinase kinases. It has been shown to inhibit the activation of p38 and JNK kinase cascades induced by environmental stresses. This phosphatase can also dephosphorylate cyclin-dependent kinases, and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to activate the expression of the tumor suppressor gene TP53/p53, which leads to G2/M cell cycle arrest and apoptosis.

Imunogênio

Purified recombinant fragment of human PPM1A expressed in E. Coli

Aplicação

Anti-PPM1A, clone 7F12 Antibody is an antibody against PPM1A for use in western blotting, flow cytometry & ELISA.

Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected PPM1A in HeLa cells.

ELISA: A representative lot detected control Antigen (100 ng) and Antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

Research Category
Signaling

Research Sub Category
Metabolic Hormones & Receptors

Qualidade

Evaluated by Western Blotting in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected PPM1A in Jurkat, A431, HeLa, Raji, MCF-7 and COS7 cell lysates.

Descrição-alvo

~43 kDa observed. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal in PBS containing up to 0.1% sodium azide and 0.5% protein stabilizer.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable