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Documentos Principais

MABS1994M

Sigma-Aldrich

Anti-MT-ND4 Antibody, clone 9E4-2D8

clone 9E4-2D8, from mouse

Sinônimo(s):

NADH-ubiquinone oxidoreductase chain 4, EC: 1.6.5.3, NADH dehydrogenase subunit 4

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

mouse

Nível de qualidade

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

9E4-2D8, monoclonal

reatividade de espécies

human

embalagem

antibody small pack of 25 μg

técnica(s)

western blot: suitable

Isotipo

IgG2aκ

nº de adesão NCBI

nº de adesão UniProt

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... MT-ND4(4538)

Descrição geral

NADH-ubiquinone oxidoreductase chain 4 (UniProt: P03905; also known as EC: 1.6.5.3, NADH dehydrogenase subunit 4, MT-ND4) is encoded by the MT-ND4 (also known as MTND4, NADH4, ND4) gene (Gene ID: 4538) in human. MT-ND4 is an inner mitochondrial membrane protein that belongs to the complex I subunit 4 family. It is a core subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I). The complex couples the oxidation of NADH and the reduction of ubiquinone (Coenzyme Q10), to the generation of a proton gradient, which is then used for ATP synthesis. The complex can be dissociated into two main sub-complexes, corresponding to the "ankle" of the boot, and the "foot" of the boot. The ankle is thought to protrude from the membrane so as to be predominantly in the aqueous phase on the matrix side. It contains the binding site for NAD(H), and the input electron transfer chain. The foot (hydrophobic) is membrane bound and contains a catalytic site at which ubiquinone is reduced. Defects in MT-ND4 gene are known to cause Leber hereditary optic neuropathy (LHON) that is characterized by acute or subacute loss of central vision due to optic nerve dysfunction. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes. Other diseases associated with MT-ND4 gene mutations are age-related macular degeneration, mesial temporal lobe epilepsy, and cystic fibrosis.

Especificidade

Clone 9E4-2D8 detects NADH-ubiquinone oxidoreductase chain 4 in mitochodria isolated from human cells.

Imunogênio

KLH-conjugated linear peptide from the C-terminal region.

Aplicação

Anti-MT-ND4, clone 9E4-2D8, Cat. No. MABS1994,is a mouse monoclonal antibody that detects NADH-ubiquinone oxidoreductase chain 4 and has been tested for use in Western Blotting.
Western Blotting Analysis: 4 µg/mL from a representative lot detected MT-ND4 in mitochondria from Human Neonatal Dermal Fibroblasts and mitochondria from Human neonatal dermal fibroblasts depleted of mtDNA. (Courtesy of Michael F. Marusich, Ph.D., mAbDx, Inc., Eugene, OR, USA).

Qualidade

Evaluated by Western Blotting in Mitochondria from human neonatal dermal fibroblasts and mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Western Blotting Analysis: 1 µg/mL of this antibody detected MT-ND4 in Mitochondria from human neonatal dermal fibroblasts and did not detect it in mitochondria from human neonatal dermal fibroblasts depleted of mtDNA.

Descrição-alvo

~37 kDa observed; 51.58 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

forma física

Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing HEPES-Buffered Saline (150 mM NaCl, 15 mM HEPES, pH 7.2) with 0.02% sodium azide.

Outras notas

Concentration: Please refer to lot specific datasheet.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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