MABN2678

Anti-phospho-α-synuclein (Ser129) Antibody, clone PS129

• Sinônimo(s): Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor (NACP)
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: PS129, monoclonal
• peso molecular: calculated mol wt 14.46 kDa; observed mol wt ~16 kDa
• purificado por: using protein G
• reatividade de espécies: human, mouse
• reatividade da espécie (prevista por homologia): bovine, porcine, rat
• embalagem: antibody small pack of 100
• técnica(s): ELISA: suitable; immunohistochemistry: suitable; western blot: suitable
• Isotipo: IgG2aκ
• sequência de epítopo: C-terminal
• nº de adesão de ID de proteína: NP_000336
• nº de adesão UniProt: P37840
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: human ... SNCA(6622)

Descrição

Especificidade

Clone PS129 is a mouse monoclonal antibody that specifically detects -synuclein phosphorylated on serine 129.

Imunogênio

KLH-conjugated linear peptide corresponding to 9 amino acids surrounding phosphoserine 129 from the C-terminal region of human -synuclein.

Aplicação

Quality Control Testing

Evaluated by Western Blotting with recombinant -synuclein phosphorylated S129 and recombinant -synuclein monomers.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected -synuclein phosphorylated on serine 129 in recombinant -synuclein phosphorylated S129 protein, but not in recombinant -synuclein monomers.

Tested Applications

ELISA Analysis: A representative lot detected -synuclein in ELISA application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104: 85-96).

Immunohistochemistry Application: A representative lot detected phospho- -synuclein (Ser129) in Immunohistochemistry application (Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99).

Western Blotting Analysis: A representative lot detected phospho- -synuclein (Ser129) in Western Blotting application (Majbour, N.K., et al. (2016). Mol Neurodegener. 11:7).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Alpha-synuclein (UniProt: P37840; also known as Non-A beta component of AD amyloid, Non-A4 component of amyloid precursor, NACP) is encoded by the SNCA (also known as NACP, PARK1) gene (Gene ID: 6622) in human. Alpha-synuclein is a membrane-bound protein in dopaminergic neurons that is involved in the regulation of dopamine release and transport. It is also reported to induce fibrillization of microtubule-associated protein tau and reduces neuronal responsiveness to various apoptotic stimuli. It is primarily expressed in the brain and is associated with the regulation of neurotransmitter release at synapses. Alpha-synuclein is also found in the cytoplasm, where it exists in a dynamic equilibrium between a soluble cytosolic form and a membrane-bound form. It associates with lipid membranes, particularly those of synaptic vesicles. Aggregation of alpha-synuclein is known to be a causative factor in the development of Parkinson′s disease (PD), which leads to the degeneration of dopaminergic neurons in the substantia nigra (SN) in the midbrain region. -synuclein can be phosphorylated, predominantly on serine residues by casein kinase 1 (CK1). Phosphorylation by CK1 appears to occur on residue (serine 129) distinct from the residue (tyrosine 125) phosphorylated by other kinase FYN. The phosphorylation of -synuclein at serine 129 (pS129) has been extensively studied due to its significant implications in neurodegenerative diseases, particularly Parkinson′s disease (PD) and other synucleinopathies. Phosphorylation of Ser129 is reported to be selective and extensive in synucleinopathy lesions and promotes insoluble fibril formation. This serves as a potential biomarker for disease diagnosis and progression and offers a promising target for therapeutic intervention. (Ref.: Vaikath, N.N., et al. (2015). Neurobiol Dis. 79:81-99; Majbour, N.K., et al. (2016). Mol Neurodegener. 19:11:7; El-Agnaf, O., et al. (2017). Neurobiol Dis. 104:85-96).

forma física

Purified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstituição

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommended storage: +2°C to +8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable