MABN2592

Anti-RBP3 Antibody, clone mAb5

• Sinônimo(s): IRBP, Interphotoreceptor retinoid-binding protein, Interstitial retinol-binding protein, Protein 7S, Retinol-binding protein 3
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: mAb5, monoclonal
• reatividade de espécies: bovine
• embalagem: antibody small pack of 100
• técnica(s): electron microscopy: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1κ
• sequência de epítopo: N-terminal half
• nº de adesão de ID de proteína: NP_776589
• nº de adesão UniProt: P12661
• Informações sobre genes: bovine ... RBP3(281443)

Descrição

Especificidade

Clone mAb5 is a mouse monoclonal antibody that detects Retinol-binding protein 3 (RBP3). It targets an epitope within the N-terminal half.

Imunogênio

Purified Retinol-binding protein 3 (RBP3) from bovine retina.

Aplicação

Quality Control Testing

Evaluated by Western Blotting in lysate from Bovine retinal tissue.

Western Blotting Analysis: A 1:500 dilution of this antibody RBP3 in lysate from Bovine retinal tissue.

Tested Applications

Electron Microscopy: A representative lot detected RBP3 in Electron Microscopy applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RBP3 in Immunoprecipitation applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Western Blotting Analysis: A representative lot detected RBP3 in Western Blotting applications (Sears, A.E., et al. (2020). FASEB J. 34(10):13918-13934).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Retinol-binding protein 3 (UniProt: P12661; also known as Interphotoreceptor retinoid-binding protein, IRBP, Interstitial retinol-binding protein, RBP3, Protein 7S) is encoded by the RBP3 gene (Gene ID: 281443) in bovine. RBP3 is synthesized with a signal peptide (aa 1-17) and a propeptide (aa 18-22) that are subsequently cleaved off to produce the mature form. It is a lipophilic glycoprotein that is secreted by rod and cone photoreceptors and plays a major role in photoreceptor homeostasis as well as retinoid and polyunsaturated fatty acid transport between the neural retina and retinal pigment epithelium. Its expression is observed early in retinal differentiation. RBP3 shuttles 11-cis and all trans retinoids between the retinol isomerase in the pigment epithelium and the visual pigments in the photoreceptor cells of the retina. It can bind many retinoids and polyunsaturated fatty acids and in addition to retinoid binding, it also plays a role in retinal development and homeostasis. RBP3 also displays thiol-dependent antioxidant activity that maintains the delicate redox balance in the normal retina. RBP3 dysregulation has been linked to retinal diseases. Mice lacking RBP3 exhibit severe early and progressive photoreceptor degeneration that is characterized by a reduction in both length and numbers of cone sheaths. In mammals four protein subunits of RBP3 have been described (M1-4), each consisting of about 300 amino acids. Clone mAb5 has been mapped to M2 subunit of RBP3. (Ref.: Sears, AE., et al. (2020) FASEB J. 34(10); 13918-13934; Zheng, S., et al. (2020). Front. Cell. Neurosci. 14; Article 577935).

forma física

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl and 0.05% sodium azide.

Reconstituição

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommended storage: +2°C to +8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable