MABN109

Anti-Synaptotagmin-4 Antibody, clone 10C11.1

clone 10C11.1, from mouse

• Sinônimo(s): Synaptotagmin-4, Synaptotagmin IV, SytIV
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 10C11.1, monoclonal
• reatividade de espécies: human, mouse
• técnica(s): immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_065834
• nº de adesão UniProt: Q9H2B2
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SYT4(6860)

Descrição

Descrição geral

Synaptotagmin-4 (UniProt Q9H2B2; also known as Synaptotagmin IV, SytIV) is encoded by the SYT4 (also known as KIAA1342) gene (Gene ID 6860) in human. The mammalian synaptotagmin family is comprised of 17 members. While most of them are predominantly function in the cetnral nervous system (CNS), some are involved in the vesicular functions of endocrine cells and glucose-transport metabolic cells. Synaptotagmin-4 is an inducible member detectable only in the brain and the neuroendocrine system. Unlike other family members, synaptotagmin-4 does not bind calcium and does not medidate calcium-induced exocytosis. Instead, synaptotagmin-4 is shown to play a neuroendocrine role in inhibiting exocytotic activities in the posterior pituitary and in cultured PC12 cells. Synaptotagmin-4 is expressed most abundantly in oxytocin neurons of the hypothalamus, where it negatively regulates oxytocin exocytosis. Dietary obesity is associated with increased vesicle binding of synaptotagmin-4 and decreased oxytocin release. Normalizing oxytocin release via Synaptotagmin-4 inhibition prevents against dietary obesity under chronic nutritional excess. Human synaptotagmin-4 consists of a vesiclular domain (a.a. 1-16), a transmembrane segment (a.a. 17-37),and a large cytoplasmic domain (a.a. 38-425).

Especificidade

Clone 10C11.1 targets a cytoplasmic epitope present in both human spliced isoforms reported by UniProt (Q9H2B2).

Imunogênio

Epitope: Cytoplasmic domain.

GST-tagged recombinant human Synaptotagmin-4 cytoplasmic domain fragment.

Aplicação

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Synaptotagmin-4 in human kidney, skeletal muscle, and pancreas tissue sections.

Research Category
Neuroscience

Research Sub Category
Vesicular Trafficking

This Anti-Synaptotagmin-4 Antibody, clone 10C11.1 is validated for use in Western Blotting, Immunohistochemistry (Paraffin) for the detection of Synaptotagmin-4.

Qualidade

Evaluated by Western Blotting in mouse cerebellum tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Synaptotagmin-4 in 10 µg of mouse cerebellum tissue lysate.

Descrição-alvo

~48 kDa observed. 47.96/46.06 kDa (human isoform 1/2) and 47.66 kDa (mouse) calculated.

forma física

Format: Purified

Protein G purified.

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1