MABF3230

Anti-CD70 Antibody, clone FR70

• Sinônimo(s): CD27 ligand, CD70 antigen, Cd27l, Cd27lg, Tnfsf7, Tumor necrosis factor ligand superfamily member 7
• Código UNSPSC: 12352203

Propriedades

• fonte biológica: rat
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: FR70, monoclonal
• peso molecular: calculated mol wt 21.92 kDa
• purificado por: using protein G
• reatividade de espécies: mouse
• embalagem: antibody small pack of 100
• técnica(s): flow cytometry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable; western blot: suitable
• Isotipo: IgG2bκ
• sequência de epítopo: Extracellular domain
• nº de adesão de ID de proteína: NP_035747
• nº de adesão UniProt: O55237
• temperatura de armazenamento: -10 to -25°C
• Informações sobre genes: mouse ... Cd70(21948)

Descrição

Especificidade

Clone FR70 is a rat monoclonal antibody that detects mouse CD70. It targets an epitope within the extracellular domain.

Imunogênio

A20 cells

Aplicação

Quality Control Testing

Evaluated by Western Blotting in mouse splenocytes lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected CD70 in mouse splenocytes lysate.

Tested Applications

Inhibition Assay: A representative lot inhibited binding of a murine CD27 Fc fusion protein to mouse CD70 in Inhibition Assays (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Flow Cytometry Analysis: A 1:500 dilution from a representative lot detected CD70 in P815 cells overexpressing mouse CD70.

Flow Cytometry Analysis: A representative lot detected CD70 in Flow Cytometry application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Western Blotting Analysis: A representative lot detected CD70 in Western Blotting application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD70 in Immunoprecipitation application (Oshima, H., et al. (1998). Int Immunol. 10(4):517-26).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

CD70 antigen (UniProt: O55237; also known as CD27 ligand, CD27-L, Tumor necrosis factor ligand superfamily member 7) is encoded by the Cd70 (also known as Cd27l, Cd27lg, Tnfsf7) gene (Gene ID: 21948) in mouse. CD70 is a type II transmembrane protein belonging to the tumor necrosis factor (TNF) family. The extracellular domain is involved in binding to its receptor, CD27, which is a member of the TNF receptor family. CD70, together with CD27, plays a critical role in the immune system and involved in T and B cell activation and differentiation and in the formation and maintenance of memory T cells, which are essential for long-term immunity. CD70 expression is tightly regulated and primarily found on activated lymphocytes and dendritic cells. Overexpression of CD70 in tumors can lead to immune evasion by inducing T cell exhaustion and apoptosis through chronic CD70-CD27 signaling. Targeting CD70 or CD27 is being explored as a therapeutic strategy in oncology. Dysregulated CD70 expression can contribute to autoimmune conditions and chronic infections as well. (Ref.: Flieswasser, T., et al., 2022, J. Exp. Clin. Cancer Res., 41:12, Oshima, H., et al. (1998). Int Immunol. 10(4):517-26)

forma física

Purified rat monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstituição

1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommended storage: +2°C to +8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable