MABF3094

Anti-BAFF/CD257 Antibody, clone 5A8

• Sinônimo(s): B-cell-activating factor, BAFF, CD257, Tumor necrosis factor ligand superfamily member 13B
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: rat
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 5A8, monoclonal
• reatividade de espécies: mouse
• embalagem: antibody small pack of 100
• técnica(s): activity assay: suitable; direct ELISA: suitable; western blot: suitable
• Isotipo: IgG1κ
• sequência de epítopo: Unknown
• nº de adesão de ID de proteína: NP_001334238
• nº de adesão UniProt: Q9WU72
• Informações sobre genes: mouse ... Klrk1(24099)

Descrição

Especificidade

Clone 5A8 is a rat monoclonal antibody that detects B-cell activating factor (BAFF/CD257).

Imunogênio

Rat RBL cells expressing full-length mouse BAFF.

Aplicação

Quality Control Testing

Evaluated by Western Blotting with recombinant murine BAFF protein .

Western Blotting Analysis (WB): A 1:500 dilution of this antibody detected recombinant murine BAFF/CD257 protein .

Tested Applications

Activity Assay: A representative lot activated mouse BAFF in a reporter cell assay, in primary B cells. (Kowalczyk-Quintas, C., et al. (2016). J Biol.Chem.291(38):19826-34; Vigolo, M., et al. (2018). Nat Commun.9(1):1199).

ELISA Analysis: A representative lot detected BAFF/CD257 in ELISA applications (Batten, M., et al. (2004). J Immunol. 172(2):812-22; Vigolo, M., et al. (2018). Nat Commun. 9(1):1199; Kowalczyk-Quintas, C., et al. (2016). J Biol Chem. 291(38):19826-34).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Tumor necrosis factor ligand superfamily member 13B (UniProt: Q9WU72; also known as B-cell-activating factor, BAFF, CD257) is encoded by the Tnfsf13b (also known as Baff) gene (Gene ID: 24099) in murine species. BAFF is a single-pass type II, homotrimeric, membrane glycoprotein with a cytoplasmic domain (aa 1-47), a transmembrane domain (aa 48-68), and an extracellular domain (aa 69-309). A soluble form of BAFF has also been described that is produced by the cleavage of membrane form between amino acids 126-127. It is expressed by cells of myeloid origin and by stromal cells. Two isoforms of BAFF have been described that are produced by alternative splicing. Isoform 2 is reported to inhibit the secretion and bioactivity of isoform 1. BAFF is reported to bind to TNFRSF13B/TACI and TNFRSF17/BCMA. It serves as a potent B cell survival factor and is a critical immune checkpoint during peripheral B cell maturation. Its levels are often elevated in autoimmune diseases, such as rheumatoid arthritis and systemic lupus erythematosus (SLE). Elevated levels of BAFF are also observed in subjects with non-Hodgkin s lymphoma (NHL) and it is reported to promote survival of both NHL-derived B lymphoma cells and B cell chronic lymphocytic leukemia (B-CLL) cells. (Ref.: Vigolo, M., et al. (2018). Nat. Commun. 9(1); 1199; Kowalczyk-Quintas, C., et al (2016). J. Biol. Chem. 291(38); 19826-19834; Batten, M., et al. (2004). J. Immunol. 172(2); 812-822; Gavin, AL., et al. (2003). J. Biol. Chem. 278(40); 38220-38228).

forma física

Purified rat monoclonal antibody IgG1 in PBS without azide.

Reconstituição

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable