MABF3054

Anti-SARS CoV-2 RBD mutant N501Y Antibody, clone 8A6

• Sinônimo(s): Spike glycoprotein Mutant N501Y
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: 8A6, monoclonal
• peso molecular: calculated mol wt 141 kDa
• purificado por: using protein G
• reatividade de espécies: vaccinia virus
• embalagem: antibody small pack of 100
• técnica(s): ELISA: suitable; western blot: suitable
• Isotipo: IgGκ
• sequência de epítopo: Extracellular domain
• nº de adesão de ID de proteína: N/A
• nº de adesão UniProt: P0DTC2
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: vaccinia virus ... S(43740568)

Descrição

Especificidade

Clone 8A6 is a mouse monoclonal antibody that detects N501Y mutant form of of SARS-CoV-2 spike protein.

Imunogênio

KLH-conjugated linear peptide segment surrounding N501Y mutant site.

Aplicação

Quality Control Testing

Evaluated by Western Blotting with recombinant SARS-CoV-2 Spike RBD (N501Y) protein .

Western Blotting Analysis: A 1:500 dilution of this antibody detected recombinant SARS-CoV-2 RBD mutant N501Y protein.

Tested Applications

ELISA Analysis: Serial dilutions of this antibody detected various recombinant SARS-CoV-2 protein samples

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Spike glycoprotein (UniProt: P0DTC2; also known as S glycoprotein, E2, Peplomer protein) is encoded by the S (also known as 2) gene (Gene ID: 43740568) in Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The SARS-CoV-2 is a positive-strand RNA virus that causes severe respiratory syndrome in human. The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S). E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The S protein is a single-pass type I, homotrimeric, membrane glycoprotein that is responsible for virus binding and entry into host cell. It is synthesized with a signal peptide (aa 1-12), which is subsequently cleaved off to generate the mature protein that contains an extracellular domain (aa 13-1213), a transmembrane domain (aa 1214-1234), and a cytoplasmic domain 1235-1273). The S protein is further processed into S1 (aa 13-685) and S2 (aa 686-1273) subunits by host cell furin. The presence of a furin polybasic cleavage site in S protein from SARS-CoV-2 sets it apart from S protein in SARS-CoV that possesses a monobasic S1/S2 cleavage site. The S1 subunit has the receptor binding domain (RBD; aa 319-541) that mediates entry of SARS-CoV-2 into sensitive cells through the peptidase domain of host Angiotensin-converting enzyme 2 (ACE2) with high affinity (KD = 15 nM). The S2 domain (aa 686-1213), which is reported to be well conserved, is responsible for membrane fusion. Over the course of the pandemic, SARS-CoV2 has consistently mutated giving rise to various mutants. Clone 8A6 recognizes SARS-CoV-2 spike protein with N501Y mutation. Several variants, including B.2.2.7, B.1.351, and P.1 share this mutation on the receptor binding domain (RBD) and is a more infectious variant. RBD with N501Y mutation exhibits a stronger interaction with ACE2 receptor and slower dissociation rate. (Ref.: Liu, Y., et al. (2022). Nature. 602(7696); 294-299; Tian, F., et al. (2021). eLife. 10; e69091).

forma física

Purified mouse monoclonal antibody IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Reconstituição

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Recommended storage: +2°C to +8°C.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable